ArticleMolecular vision2010
The expression of Wnt2b in the optic cup lip requires a border between the pigmented and nonpigmented epithelium.
Article in Molecular vision, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- The development and the genetic diseases of the ciliary body.Cell insight · 2024Review
- BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling.Biology open · 2017Article
- Establishment of the neurogenic boundary of the mouse retina requires cooperation of SOX2 and WNT signaling.Neural development · 2014Article
- Article
- Identification of Wnt/β-catenin modulated genes in the developing retina.Molecular vision · 2012Article
- Retinal and anterior eye compartments derive from a common progenitor pool in the avian optic cup.Molecular vision · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeWnt2b is normally expressed at the optic cup lip and is implicated in ciliary body induction. The lens has often been considered an organizer for the anterior eye, but recent studies demonstrate that the anterior cell fates are correctly specified in the absence of the lens. This study uses Wnt2b as a marker to reveal the mechanism behind the specification of the anterior domain of the optic cup.
methodsDeveloping chick embryos were used as a model system. Eyes were microsurgically manipulated to assess the role of the lens in the development of the anterior optic cup. Eyes were molecularly manipulated, using fibroblast growth factor expressing replication-incompetent retrovirus, introduced into the retinal pigmented epithelium (RPE) domain. Ectopic fibroblast growth factor transformed the RPE into nonpigmented epithelium (NPE; ciliary body). As the virus does not spread, discrete borders between RPE and NPE were experimentally created. Wnt2b expression was assessed after surgical and molecular manipulation.
resultsContrary to expectations, we found that the lens is not able to induce Wnt2b expression in optic cup tissue: When the optic cup lip is experimentally misspecified such that it no longer contains the juxtaposition of pigmented and nonpigmented tissue, Wnt2b is not expressed. In addition, if the prelens ectoderm is removed from the optic vesicle before morphogenesis, the resulting lensless optic cup expresses Wnt2b even though it was not in contact with lens tissue. We also show that ectopic lenses do not induce Wnt2b in optic cup tissue. The ciliary body/anterior eye domain is specified at the border of RPE and the NPE of the ciliary body. During development, this border is normally found at the optic cup lip. We can manipulate tissue specification using retroviral-mediated gene transfer, and create ectopic borders between nonpigmented and pigmented tissue. At such borders, Wnt2b is ectopically expressed in the absence of lens contact. Finally, we describe a role for the lens in maintenance of Wnt2b expression and demonstrate support for this in two ways: First, we show that if the lens is removed from the formed optic cup, endogenous Wnt2b expression is specifically lost from the optic cup lip; and second, we show that while ectopic Wnt2b expression is initially found in the majority of ectopic borders, as eye development proceeds ectopic expression is maintained only in those borders that are close to the lens.
conclusionsTaken together, the results provide support for a model in which the anterior optic cup domain, as described in part by Wnt2b expression, is specified through the elaboration of a border within the optic neuroepithelium rather than through interactions with the surrounding environment.
Indexed as
Identifiers
21179237PMC3002962W77744396What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.