ArticlePloS one2010
UEV-1 is an ubiquitin-conjugating enzyme variant that regulates glutamate receptor trafficking in C. elegans neurons.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
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- A Proteome-Wide Effect of PHF8 Knockdown on Cortical Neurons Shows Downregulation of Parkinson's Disease-Associated Protein Alpha-Synuclein and Its Interactors.Biomedicines · 2023Article
- Remodeling without destruction: non-proteolytic ubiquitin chains in neural function and brain disorders.Molecular psychiatry · 2021Review
- The ubiquitin-editing enzyme A20 regulates synapse remodeling and efficacy.Brain research · 2020Article
- Transcriptomic Analysis ofFrontiers in psychiatry · 2019Article
- Proteasome-independent polyubiquitin linkage regulates synapse scaffolding, efficacy, and plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The PHR proteins: intracellular signaling hubs in neuronal development and axon degeneration.Neural development · 2016Review
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- rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.PloS one · 2016Article
- DLK-1/p38 MAP Kinase Signaling Controls Cilium Length by Regulating RAB-5 Mediated Endocytosis in Caenorhabditis elegans.PLoS genetics · 2015Article
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- The ubiquitin proteasome system in Caenorhabditis elegans and its regulation.Redox biology · 2014Review
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- Role of the ubiquitin-proteasome system in nervous system function and disease: using C. elegans as a dissecting tool.Cellular and molecular life sciences : CMLS · 2012Review
- Identification of the ubiquitin ligase Triad1 as a regulator of endosomal transport.Biology open · 2012Article
- A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting.Molecular biology of the cell · 2012Article
- Ubc13 and COOH terminus of Hsp70-interacting protein (CHIP) are required for growth hormone receptor endocytosis.The Journal of biological chemistry · 2012Article
- Ubiquitination is involved in secondary growth, not initial formation of polyglutamine protein aggregates in C. elegans.BMC cell biology · 2012Article
- Hypoxia regulates glutamate receptor trafficking through an HIF-independent mechanism.The EMBO journal · 2012Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The regulation of AMPA-type glutamate receptor (AMPAR) membrane trafficking is a key mechanism by which neurons regulate synaptic strength and plasticity. AMPAR trafficking is modulated through a combination of receptor phosphorylation, ubiquitination, endocytosis, and recycling, yet the factors that mediate these processes are just beginning to be uncovered. Here we identify the ubiquitin-conjugating enzyme variant UEV-1 as a regulator of AMPAR trafficking in vivo. We identified mutations in uev-1 in a genetic screen for mutants with altered trafficking of the AMPAR subunit GLR-1 in C. elegans interneurons. Loss of uev-1 activity results in the accumulation of GLR-1 in elongated accretions in neuron cell bodies and along the ventral cord neurites. Mutants also have a corresponding behavioral defect--a decrease in spontaneous reversals in locomotion--consistent with diminished GLR-1 function. The localization of other synaptic proteins in uev-1-mutant interneurons appears normal, indicating that the GLR-1 trafficking defects are not due to gross deficiencies in synapse formation or overall protein trafficking. We provide evidence that GLR-1 accumulates at RAB-10-containing endosomes in uev-1 mutants, and that receptors arrive at these endosomes independent of clathrin-mediated endocytosis. UEV-1 homologs in other species bind to the ubiquitin-conjugating enzyme Ubc13 to create K63-linked polyubiquitin chains on substrate proteins. We find that whereas UEV-1 can interact with C. elegans UBC-13, global levels of K63-linked ubiquitination throughout nematodes appear to be unaffected in uev-1 mutants, even though UEV-1 is broadly expressed in most tissues. Nevertheless, ubc-13 mutants are similar in phenotype to uev-1 mutants, suggesting that the two proteins do work together to regulate GLR-1 trafficking. Our results suggest that UEV-1 could regulate a small subset of K63-linked ubiquitination events in nematodes, at least one of which is critical in regulating GLR-1 trafficking.
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