ArticleMolecular systems biology2010
Defining the budding yeast chromatin-associated interactome.
Article in Molecular systems biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- Lysine demethylase 4A: from chromatin to centrosomes.The FEBS journal · 2026Article
- ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.Nucleic acids research · 2023Article
- Human transcription factor protein interaction networks.Nature communications · 2022Article
- ATAD2 controls chromatin-bound HIRA turnover.Life science alliance · 2021Article
- The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity.Nature communications · 2021Article
- Human Histone Interaction Networks: An Old Concept, New Trends.Journal of molecular biology · 2021Article
- CAF-1 and Rtt101p function within the replication-coupled chromatin assembly network to promote H4 K16ac, preventing ectopic silencing.PLoS genetics · 2020Article
- A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, fromBio-protocol · 2020Article
- The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion.The Journal of cell biology · 2018Article
- Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells.Journal of visualized experiments : JoVE · 2018Article
- Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2.Microbial cell (Graz, Austria) · 2017Article
- Proteomics to study DNA-bound and chromatin-associated gene regulatory complexes.Human molecular genetics · 2016Article
- Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.Journal of molecular cell biology · 2016Article
- HyCCAPP as a tool to characterize promoter DNA-protein interactions in Saccharomyces cerevisiae.Genomics · 2016Article
- Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes.Journal of proteomics · 2015Article
- BioTAP-XL: Cross-linking/Tandem Affinity Purification to Study DNA Targets, RNA, and Protein Components of Chromatin-Associated Complexes.Current protocols in molecular biology · 2015Article
- Article
- Advanced methods for the analysis of chromatin-associated proteins.Physiological genomics · 2014Review
- Incorporating DNA shearing in standard affinity purification allows simultaneous identification of both soluble and chromatin-bound interaction partners.Journal of proteomics · 2014Article
- Regulation of histone gene transcription in yeast.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported a novel affinity purification (AP) method termed modified chromatin immunopurification (mChIP), which permits selective enrichment of DNA-bound proteins along with their associated protein network. In this study, we report a large-scale study of the protein network of 102 chromatin-related proteins from budding yeast that were analyzed by mChIP coupled to mass spectrometry. This effort resulted in the detection of 2966 high confidence protein associations with 724 distinct preys. mChIP resulted in significantly improved interaction coverage as compared with classical AP methodology for ∼75% of the baits tested. Furthermore, mChIP successfully identified novel binding partners for many lower abundance transcription factors that previously failed using conventional AP methodologies. mChIP was also used to perform targeted studies, particularly of Asf1 and its associated proteins, to allow for a understanding of the physical interplay between Asf1 and two other histone chaperones, Rtt106 and the HIR complex, to be gained.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.