ArticleMolecular systems biology2010
A tunable dual-promoter integrator for targeting of cancer cells.
Article in Molecular systems biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Modular Scalable Synthetic Gene Circuits for Complex Functions Within Minimal Computational Layers in Human Cells.Nature communications · 2026Article
- Current Progress and Future Outlook for Synthetic Gene Circuits in Cardiovascular Therapy.Biomolecules · 2026Review
- Synthetic biology approaches to enhance cancer immune responses.Frontiers in immunology · 2025Review
- Synthetic biology approaches for improving the specificity and efficacy of cancer immunotherapy.Cellular & molecular immunology · 2024Review
- Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems.Nature communications · 2024Article
- Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells.Cell research · 2024Article
- Israel and the global synthetic biology ecosystem.Engineering biology · 2023Review
- DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron.ACS synthetic biology · 2022Article
- Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors.Nature communications · 2022Article
- Immunoregulatory framework and the role of miRNA in the pathogenesis of NSCLC - A systematic review.Frontiers in oncology · 2022Review
- Engineering living therapeutics with synthetic biology.Nature reviews. Drug discovery · 2021Review
- Synthetic Circuit-Driven Expression of Heterologous Enzymes for Disease Detection.ACS synthetic biology · 2021Article
- Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors.Science advances · 2021Article
- Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.ACS synthetic biology · 2021Article
- Implementing Logic Gates for Safer Immunotherapy of Cancer.Frontiers in immunology · 2021Review
- Design of Multipartite Transcription Factors for Multiplexed Logic Genome Integration Control in Mammalian Cells.ACS synthetic biology · 2020Article
- The intersectional genetics landscape for humans.GigaScience · 2020Article
- Synthetic metabolic computation in a bioluminescence-sensing system.Nucleic acids research · 2019Article
- A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS).Nature communications · 2019Article
- Engineering advanced cancer therapies with synthetic biology.Nature reviews. Cancer · 2019Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise discrimination between similar cellular states is essential for autonomous decision-making scenarios, such as in vivo targeting of diseased cells. Discrimination could be achieved by delivering an effector gene expressed under a highly active context-specific promoter. Yet, a single-promoter approach has linear response and offers limited control of specificity and efficacy. Here, we constructed a dual-promoter integrator, which expresses an effector gene only when the combined activity of two internal input promoters is high. A tunable response provides flexibility in choosing promoter inputs and effector gene output. Experiments using one premalignant and four cancer cell lines, over a wide range of promoter activities, revealed a digital-like response of input amplification following a sharp activation threshold. The response function is cell dependent with its overall magnitude increasing with degree of malignancy. The tunable digital-like response provides robustness, acts to remove input noise minimizing false-positive identification of cell states, and improves targeting precision and efficacy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.