ArticleInvestigative ophthalmology & visual science2011
Isolation and propagation of mesenchymal stem cells from the lacrimal gland.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- Epithelial and mesenchymal progenitor cells in normal and inflamed human lacrimal glands.Experimental eye research · 2025Article
- Human Lacrimal Gland Derived Mesenchymal Stem Cells - Isolation, Propagation, and Characterization.Investigative ophthalmology & visual science · 2023Article
- Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.Translational vision science & technology · 2022Article
- Current approaches for the regeneration and reconstruction of ocular surface in dry eye.Frontiers in medicine · 2022Review
- Systemic Treatment of Immune-Mediated Keratoconjunctivitis Sicca with Allogeneic Stem Cells Improves the Schirmer Tear Test Score in a Canine Spontaneous Model of Disease.Journal of clinical medicine · 2021Article
- Establishment of functional epithelial organoids from human lacrimal glands.Stem cell research & therapy · 2021Article
- Honey-incorporated nanofibre reduces replicative senescence of umbilical cord-derived mesenchymal stem cells.IET nanobiotechnology · 2020Article
- MSC Transplantation Improves Lacrimal Gland Regeneration after Surgically Induced Dry Eye Disease in Mice.Scientific reports · 2019Article
- Establishing and characterizing lacrispheres from human lacrimal gland for potential clinical application.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
- Lacrimal gland development: From signaling interactions to regenerative medicine.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Review
- Strategies for Regenerating the Lacrimal Gland.Current ophthalmology reports · 2017Article
- Lacrimal Gland Repair after Short-term Obstruction of Excretory Duct in Rabbits.Scientific reports · 2017Article
- Evaluation of Accessory Lacrimal Gland in Muller's Muscle Conjunctival Resection Specimens for Precursor Cell Markers and Biological Markers of Dry Eye Disease.Current eye research · 2017Article
- Molecular Profiling of the Developing Lacrimal Gland Reveals Putative Role of Notch Signaling in Branching Morphogenesis.Investigative ophthalmology & visual science · 2017Article
- Delivery of Bone Marrow-Derived Mesenchymal Stem Cells Improves Tear Production in a Mouse Model of Sjögren's Syndrome.Stem cells international · 2017Article
- Lacrimal Gland Repair Using Progenitor Cells.Stem cells translational medicine · 2017Article
- A Ligation of the Lacrimal Excretory Duct in Mouse Induces Lacrimal Gland Inflammation with Proliferative Cells.Stem cells international · 2017Article
- Myoepithelial Cells: Their Origin and Function in Lacrimal Gland Morphogenesis, Homeostasis, and Repair.Current molecular biology reports · 2015Article
- Bioengineered Lacrimal Gland Organ Regeneration in Vivo.Journal of functional biomaterials · 2015Article
- Human lacrimal gland regeneration: Perspectives and review of literature.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposePreviously, it was reported that the murine lacrimal gland is capable of repair after experimentally induced injury and that the number of stem/progenitor cells was increased during the repair phase (2-3 days after injury). The aim of the present study was to determine whether these cells can be isolated from the lacrimal gland and propagated in vitro.
methodsLacrimal gland injury was induced by injection of interleukin (IL)-1, and injection of saline vehicle served as control. Two and half days after injection, the lacrimal glands were removed and used to prepare explants or acinar cells for tissue culture. Cells derived from the explants and the acinar cells were grown in DMEM supplemented with 10% fetal bovine serum. Cells were stained for the stem cells markers, nestin, vimentin, ABCG2, and Sca-1. Cell proliferation was measured using an antibody against Ki67 or a cell-counting kit. The adipogenic capability of these cells was also tested in vitro.
resultsResults show that nestin-positive cells can be isolated from IL-1-injected, but not saline-injected, lacrimal glands. A population of nestin-positive cells was also positive for vimentin, an intermediate filament protein expressed by mesenchymal cells. In addition, cultured cells expressed two other markers of stem cells, ABCG2 and Sca-1. These cells proliferated in vitro and can be induced to form adipocytes, attesting to their mesenchymal stem cell property.
conclusionsMurine lacrimal glands contain mesenchymal stem cells that seem to play a pivotal role in tissue repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.