ArticleGenome research2011
The Drosophila melanogaster transcriptome by paired-end RNA sequencing.
Article in Genome research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed.
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- Gene expression variation across genetically identical individuals predicts reproductive traits.eLife · 2025Article
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- A distinct isoform of Msp300 (nesprin) organizes the perinuclear microtubule-organizing center in adipocytes.Molecular biology of the cell · 2025Article
- Cell-specific genetic expression profile of antennal glia in Drosophila reveals candidate genes in neuron-glia interactions.Scientific reports · 2025Article
- Identification of Splicing Variation Associated with Parental Behavior in the Burying Beetles (Journal of genomics · 2025Article
- EFFICIENT RIBOSOMAL RNA DEPLETION FROMWellcome open research · 2025Article
- RNA-seq analysis reveals changes in mRNA expression during development in Daphnia mitsukuri.BMC genomics · 2024Article
- Internal and external normalization of nascent RNA sequencing run-on experiments.BMC bioinformatics · 2024Article
- Global Gene Expression Analysis Reveals Complex Cuticle Organization of the Tribolium Compound Eye.Genome biology and evolution · 2023Article
- Recapitulation of the embryonic transcriptional program in holometabolous insect pupae.Scientific reports · 2022Article
- Alternative transcript splicing regulates UDP-glucosyltransferase-catalyzed detoxification of DIMBOA in the fall armyworm (Spodoptera frugiperda).Scientific reports · 2022Article
- Using FlyBase: A Database of Drosophila Genes and Genetics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- On the Origin and Evolution ofGenes · 2021Article
- Genome-wide evolution of wobble base-pairing nucleotides of branchpoint motifs with increasing organismal complexity.RNA biology · 2020Article
- Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).Scientific reports · 2019Article
- Combining next-generation sequencing and single-molecule sequencing to explore brown plant hopper responses to contrasting genotypes of japonica rice.BMC genomics · 2019Article
- Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
RNA-seq was used to generate an extensive map of the Drosophila melanogaster transcriptome by broad sampling of 10 developmental stages. In total, 142.2 million uniquely mapped 64-100-bp paired-end reads were generated on the Illumina GA II yielding 356× sequencing coverage. More than 95% of FlyBase genes and 90% of splicing junctions were observed. Modifications to 30% of FlyBase gene models were made by extension of untranslated regions, inclusion of novel exons, and identification of novel splicing events. A total of 319 novel transcripts were identified, representing a 2% increase over the current annotation. Alternate splicing was observed in 31% of D. melanogaster genes, a 38% increase over previous estimations, but significantly less than that observed in higher organisms. Much of this splicing is subtle such as tandem alternate splice sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.