ArticleJournal of clinical microbiology2011
Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 2 syntheses or guidelines pooled it, 32 citations in OpenAlex.
- Accuracy of reverse-transcription polymerase chain reaction and loop-mediated isothermal amplification in diagnosing severe fever with thrombocytopenia syndrome: A systematic review and meta-analysis.Journal of medical virology · 2022Pooled it
- Diagnostic value of loop-mediated isothermal amplification assay for hand, foot, and mouth disease.Journal of clinical laboratory analysis · 2021Pooled it
- Identification of enterovirus 71 hand, foot and mouth disease from other enteroviruses by microfluidic digital PCR.BMC infectious diseases · 2025Article
- Isothermal nucleic acid amplification for monitoring hand-foot-and-mouth disease: current status and future implications.Mikrochimica acta · 2024Review
- Rapid detection of four major HFMD-associated enteroviruses by multiplex HiFi-LAMP assays.Analytical and bioanalytical chemistry · 2024Article
- Development of Loop-Mediated Isothermal Amplification Method for Rapid and Sensitive Identification ofMethods and protocols · 2023Article
- Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus.Journal of microbiology (Seoul, Korea) · 2020Article
- Colorimetric biosensors for point-of-care virus detections.Materials science for energy technologies · 2020Review
- Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.The Journal of antimicrobial chemotherapy · 2015Review
- Improved detection limit in rapid detection of human enterovirus 71 and coxsackievirus A16 by a novel reverse transcription-isothermal multiple-self-matching-initiated amplification assay.Journal of clinical microbiology · 2014Article
- Detection of new bunyavirus RNA by reverse transcription-loop-mediated isothermal amplification.Journal of clinical microbiology · 2014Article
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- Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification.Virology journal · 2011Article
- Simple and rapid detection of human enterovirus 71 by reverse-transcription and loop-mediated isothermal amplification: cryopreservation affected the detection ability.Diagnostic microbiology and infectious disease · 2011Article
- External quality assessment for enterovirus 71 and coxsackievirus A16 detection by reverse transcription-PCR using armored RNA as a virus surrogate.Journal of clinical microbiology · 2011Article
- Development and evaluation of reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71.BMC infectious diseases · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enterovirus 71 (EV71) is the major etiological agent of hand, foot, and mouth disease (HFMD), which is a common infectious disease in young children and infants. EV71 can cause various clinical manifestations and has been associated with severe neurological complications; it has resulted in fatalities during recent outbreaks in Asian-Pacific regions since 1997. The early and rapid detection is critical for prevention and control of EV71 infection, since no vaccine or antiviral drugs are currently available. In this study, a simple and sensitive reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid detection of EV71. The detection limit of the RT-LAMP assay was approximately 0.01 PFU per reaction mixture, and no cross-reactive amplification with other enteroviruses was observed. The assay was evaluated further with 40 clinical specimens and exhibited 92.9% sensitivity and 100% specificity. This RT-LAMP assay may become a useful alternative in clinical diagnosis of EV71, especially in resource-limited hospitals or rural clinics of China and other countries in the Asian-Pacific region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.