ArticleJournal of clinical microbiology2011
Novel pan-genomic analysis approach in target selection for multiplex PCR identification and detection of Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia cepacia complex species: a proof-of-concept study.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Identification ofMicrobiology spectrum · 2024Article
- Detection ofFrontiers in public health · 2023Article
- Reliable detection of Burkholderia pseudomallei using multiple cross displacement amplification label-based biosensor.BMC microbiology · 2022Article
- Detection and differentiation of Burkholderia species with pathogenic potential in environmental soil samples.PloS one · 2021Article
- A Phylogeny-Informed Proteomics Approach for Species Identification within the Burkholderia cepacia Complex.Journal of clinical microbiology · 2020Article
- Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens.International journal of molecular sciences · 2020Article
- Small Noncoding Regulatory RNAs fromInternational journal of molecular sciences · 2018Review
- Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos.Scientific reports · 2018Article
- Article
- The Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers.mBio · 2016Article
- Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis.International journal of molecular sciences · 2016Article
- Article
- The bacterial pangenome as a new tool for analysing pathogenic bacteria.New microbes and new infections · 2015Article
- Evaluation of Molecular Methods To Improve the Detection of Burkholderia pseudomallei in Soil and Water Samples from Laos.Applied and environmental microbiology · 2015Article
- Laboratory diagnosis of melioidosis: past, present and future.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway.Cell & bioscience · 2015Article
- Analysis of changes in diversity and abundance of the microbial community in a cystic fibrosis patient over a multiyear period.Journal of clinical microbiology · 2015Article
- Burkholderia pseudomallei in soil samples from an oceanarium in Hong Kong detected using a sensitive PCR assay.Emerging microbes & infections · 2014Article
- An efficient alternative marker for specific identification of Mycobacterium tuberculosis.World journal of microbiology & biotechnology · 2014Article
- Fatal melioidosis in goats in Bangkok, Thailand.The American journal of tropical medicine and hygiene · 2014Article
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9 authors.
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Abstract
Burkholderia pseudomallei, Burkholderia thailandensis, and the Burkholderia cepacia complex differ greatly in pathogenicity and epidemiology. Yet, they are occasionally misidentified by biochemical profiling, and even 16S rRNA gene sequencing may not offer adequate discrimination between certain species groups. Using the 23 B. pseudomallei, four B. thailandensis, and 16 B. cepacia complex genome sequences available, we identified gene targets specific to each of them (a Tat domain protein, a 70-kDa protein, and a 12-kDa protein for B. pseudomallei, B. thailandensis, and the B. cepacia complex, respectively), with an in-house developed algorithm. Using these targets, we designed a robust multiplex PCR assay useful for their identification and detection from soil and simulated sputum samples. For all 43 B. pseudomallei, seven B. thailandensis, and 20 B. cepacia complex (B. multivorans, n = 6; B. cenocepacia, n = 3; B. cepacia, n = 4; B. arboris, n = 2; B. contaminans, B. anthina, and B. pyrrocinia, n = 1 each; other unnamed members, n = 2) isolates, the assay produced specific products of predicted size without false positives or negatives. Of the 60 soil samples screened, 19 (31.6%) and 29 (48.3%) were positive for B. pseudomallei and the B. cepacia complex, respectively, and in four (6.7%) soil samples, the organisms were codetected. DNA sequencing confirmed that all PCR products originated from their targeted loci. This novel pan-genomic analysis approach in target selection is simple, computationally efficient, and potentially applicable to any species that harbors species-specific genes. A multiplex PCR assay for rapid and accurate identification and detection of B. pseudomallei, B. thailandensis, and the B. cepacia complex was developed and verified.
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