ArticleJournal of clinical microbiology2011
Detection and differentiation of Cryptosporidium spp. in human clinical samples by use of real-time PCR.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
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- Genome-targeted enrichment and sequencing of human-infecting Cryptosporidium spp.Nature communications · 2026Article
- Evaluation of the Biosynex AMPLIQUICKFrontiers in cellular and infection microbiology · 2026Article
- Cryptosporidium Diagnostic Assays: Molecular Detection and Species Determination by Real-Time PCR-Based Assays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Mouse Models for Use in Cryptosporidium Infection Studies, Quantification of Parasite Burden Using Flow Cytometry, qPCR and Histopathology, and Confocal Imaging of Oocysts.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Epidemiology of Human Cryptosporidiosis in Brazil: A Systematic Review HighlightingTropical medicine and infectious disease · 2025Review
- Meta-Transcriptomic Response to Copper Corrosion in Drinking Water Biofilms.Microorganisms · 2025Article
- Molecular and Epidemiological Investigation ofPathogens (Basel, Switzerland) · 2025Article
- Endoparasites of European hedgehogs (Erinaceus europaeus) in Germany and their zoonotic potential: proposed Capillaria ovoreticulata genetically identified as Capillaria putorii.Parasites & vectors · 2025Article
- Detection ofFood and waterborne parasitology · 2025Article
- Harnessing Non-standard Nucleic Acids for Highly Sensitive Icosaplex (20-Plex) Detection of Microbial Threats for Environmental Surveillance.ACS synthetic biology · 2025Article
- Cryptosporidiosis: A global threat to human and animal health.Open veterinary journal · 2025Review
- Genetic Characterization and Zoonotic Potential ofTransboundary and emerging diseases · 2025Article
- Investigating genetic diversity withinCurrent research in parasitology & vector-borne diseases · 2025Article
- Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogenCurrent research in parasitology & vector-borne diseases · 2025Article
- Evaluation of the Allplex GI Parasite and Helminth PCR Assay in a Belgian Travel Clinic.Diagnostics (Basel, Switzerland) · 2024Article
- Assessment of the potential occurrence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Anti-Cryptosporidium oocysts polyclonal antibodies for cryptosporidiosis diagnosis and protection.AMB Express · 2023Article
- The occurrence and zoonotic potential of Cryptosporidium species in freshwater biota.Parasites & vectors · 2023Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time PCR has the potential to streamline detection and identification of Cryptosporidium spp. in human clinical samples. In the present article, we report the first such assay to allow not only detection and differentiation of the most common human pathogens, Cryptosporidium hominis and Cryptosporidium parvum, but also simultaneous amplification of a region of the small subunit (SSU) rRNA gene, permitting direct sequence analysis to identify any Cryptosporidium species. An internal control is incorporated to identify the presence of PCR inhibitors. Analytical sensitivity was determined to be as low as 200 oocysts per gram of feces processed, equivalent to 2 oocysts per PCR. The C. hominis and C. parvum PCRs specifically detected only species/genotypes in their respective target clades. Diagnostic sensitivity and specificity, evaluated against a widely used conventional nested SSU rRNA gene PCR as a nominated gold standard using a panel of 258 (151 positive and 107 negative) samples, were 100% and 99.1%, respectively. The assay agreed with PCR-restriction fragment length polymorphism analysis of the Cryptosporidium oocyst wall protein gene for 134 of 136 (98.5%) samples tested prospectively and typed two additional isolates. The real-time PCR assay was sensitive, specific, and reproducible and significantly improved laboratory work flow and turnaround times.
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