ArticleJournal of clinical microbiology2011
Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers, 2 of them syntheses that pooled it.
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Who cites it
122 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Association between Blastocystis sp. infection and immunocompromised patients: a systematic review and meta-analysis.Environmental science and pollution research international · 2021Pooled it
- Epidemiology of Blastocystis sp. infection in China: a systematic review.Parasite (Paris, France) · 2019Pooled it
- MappingOne health (Amsterdam, Netherlands) · 2026Article
- Molecular Detection of Enteric Protozoa in Cattle from North Portugal.Pathogens (Basel, Switzerland) · 2026Article
- First survey on the occurrence, subtype distribution and circulation of the enteric protozoan Blastocystis sp. in French broiler chicken farms.Poultry science · 2026Article
- Occurrence and subtyping of the intestinal protozoanFood and waterborne parasitology · 2026Article
- MultipleFood and waterborne parasitology · 2025Article
- Assessment of the Therapeutic Role of Allium tuncelianum Extract in Rats Infected with Blastocystis Subtype 3.Acta parasitologica · 2025Article
- Molecular Identification ofJournal of clinical medicine · 2025Article
- Improvement of the diagnosis of intestinal protozoa using a multiplex qPCR strategy compared to classical microscopy: a prospective study on 3,500 stool samples over 3 years.Journal of clinical microbiology · 2025Article
- Is Parasitic Contamination of Soil in the Southern United States Related to Poverty and Does It Represent a Human Health Threat? A Perspective.The American journal of tropical medicine and hygiene · 2025Article
- Targeted-Amplicon NGS forVeterinary sciences · 2025Article
- AnthropogenicMicroorganisms · 2025Article
- Investigation ofAfrican health sciences · 2025Article
- First Report on the Frequency and Subtype Distribution ofAnimals : an open access journal from MDPI · 2025Article
- Molecular detection of zoonoticalFrontiers in veterinary science · 2025Article
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- Current status of intestinal parasitosis and microsporidiosis in industrialized countries: Results from a prospective study in France and Luxembourg.PLoS neglected tropical diseases · 2024Article
- Blastocystis hominis; Friend or Foe.Integrative medicine (Encinitas, Calif.) · 2024Review
62 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocystis anaerobic parasites are widespread worldwide in the digestive tract of many animal species, including humans. Epidemiological Blastocystis studies are often limited by the poor sensitivity of standard parasitological assays for its detection. This report presents a highly sensitive real-time quantitative PCR (qPCR) assay developed to detect Blastocystis parasites in stool samples. The assay targets a partial sequence of the Blastocystis small ribosomal subunit (SSU) rRNA gene, allowing subtyping (ST) of Blastocystis isolates by direct sequencing of qPCR products. This qPCR method was assessed in a prospective study of 186 patients belonging to two cohorts--a group of 94 immunocompromised patients presenting hematological malignancies and a control group of 92 nonimmunocompromised patients. Direct-light microscopy and xenic in vitro stool culture analysis showed only 29% and 52% sensitivity, respectively, compared to our qPCR assay. Of the 27 (14.5%) Blastocystis-positive patients, 8 (4%) experienced digestive symptoms. No correlation was found between symptomatic patients and immune status, parasite load, or parasite subtypes, although subtyping of all isolates revealed a high (63.0%) prevalence of ST4. Two unexpected avian subtypes were found, i.e., ST6 and ST7, which are frequently isolated in Asia but rarely present in Western countries. In conclusion, this qPCR proved by far the most sensitive of the tested methods and allowed subtype determination by direct sequencing of qPCR products. New diagnostic tools such as the qPCR are essential for evaluating the clinical relevance of Blastocystis subtypes and their role in acute or chronic digestive disorders.
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