ArticleJournal of clinical microbiology2011
Identification of Pseudallescheria and Scedosporium species by three molecular methods.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
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- Diagnosis and management of invasive fungal infections due to non-Aspergillus moulds.The Journal of antimicrobial chemotherapy · 2025Review
- First environmental survey ofFrontiers in cellular and infection microbiology · 2025Article
- An updated list of eumycetoma causative agents and their differences in grain formation and treatment response.Clinical microbiology reviews · 2024Review
- Descriptions of 19 Unrecorded Species Belonging toMycobiology · 2024Article
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- Fungal Genomics in Respiratory Medicine: What, How and When?Mycopathologia · 2021Review
- Phenomic profiling of a novel sibling species within the Scedosporium complex in Thailand.BMC microbiology · 2021Article
- Madurella real-time PCR, a novel approach for eumycetoma diagnosis.PLoS neglected tropical diseases · 2020Article
- Article
- Dual DNA Barcoding for the Molecular Identification of the Agents of Invasive Fungal Infections.Frontiers in microbiology · 2019Article
- Recent Advances in the Treatment of Scedosporiosis and Fusariosis.Journal of fungi (Basel, Switzerland) · 2018Review
- Article
- New Panfungal Real-Time PCR Assay for Diagnosis of Invasive Fungal Infections.Journal of clinical microbiology · 2016Article
- Environmental Screening for the Scedosporium apiospermum Species Complex in Public Parks in Bangkok, Thailand.PloS one · 2016Article
- Unpredictable susceptibility of emerging clinical moulds to tri-azoles: review of the literature and upcoming challenges for mould identification.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Review
- Fungal septal abscess complicating maxillary sinus fungus balls in an immunocompetent host.Allergy & rhinology (Providence, R.I.) · 2015Article
- Susceptibility and diversity in the therapy-refractory genus scedosporium.Antimicrobial agents and chemotherapy · 2014Article
- Merits and pitfalls of currently used diagnostic tools in mycetoma.PLoS neglected tropical diseases · 2014Review
- Emerging invasive fungal diseases in transplantation.Current infectious disease reports · 2012Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major clinically relevant species in Scedosporium (teleomorph Pseudallescheria) are Pseudallescheria boydii, Scedosporium aurantiacum, Scedosporium apiospermum, and Scedosporium prolificans, while Pseudallescheria minutispora, Petriellopsis desertorum, and Scedosporium dehoogii are exceptional agents of disease. Three molecular methods targeting the partial β-tubulin gene were developed and evaluated to identify six closely related species of the S. apiospermum complex using quantitative real-time PCR (qPCR), PCR-based reverse line blot (PCR-RLB), and loop-mediated isothermal amplification (LAMP). qPCR was not specific enough for the identification of all species but had the highest sensitivity. The PCR-RLB assay was efficient for the identification of five species. LAMP distinguished all six species unambiguously. The analytical sensitivities of qPCR, PCR-RLB, and LAMP combined with MagNAPure, CTAB (cetyltrimethylammonium bromide), and FTA filter (Whatman) extraction were 50, 5 × 10(3), and 5 × 10(2) cells/μl, respectively. When LAMP was combined with a simplified DNA extraction method using an FTA filter, identification to the species level was achieved within 2 h, including DNA extraction. The FTA-LAMP assay is therefore recommended as a cost-effective, simple, and rapid method for the identification of Scedosporium species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.