ArticleThe Journal of biological chemistry2011
Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance inMicrobial cell (Graz, Austria) · 2026Article
- The interactome of the Bakers' yeast peroxiredoxin Tsa1 implicates it in redox regulation of intermediary metabolism, glycolysis, and zinc homeostasis.Metallomics : integrated biometal science · 2025Article
- Discovery of metal-binding proteins by thermal proteome profiling.Nature chemical biology · 2024Article
- Zinc homeostasis in Schizosaccharomyces pombe.Archives of microbiology · 2023Review
- Zinc at the Host-Fungus Interface: How to Uptake the Metal?Journal of fungi (Basel, Switzerland) · 2020Review
- Interaction between Polyphenolic Antioxidants andBiomolecules · 2020Article
- Transcription factors and transporters in zinc homeostasis: lessons learned from fungi.Critical reviews in biochemistry and molecular biology · 2020Review
- The Loz1 transcription factor from Schizosaccharomyces pombe binds to Loz1 response elements and represses gene expression when zinc is in excess.Molecular microbiology · 2019Article
- A Role for Zinc in Plant Defense Against Pathogens and Herbivores.Frontiers in plant science · 2019Review
- Metals in fungal virulence.FEMS microbiology reviews · 2018Review
- The Zap1 transcriptional activator negatively regulates translation of the RTC4 mRNA through the use of alternative 5' transcript leaders.Molecular microbiology · 2017Article
- Zinc sensing and regulation in yeast model systems.Archives of biochemistry and biophysics · 2016Review
- Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth.The Journal of biological chemistry · 2016Article
- Cellular sensing and transport of metal ions: implications in micronutrient homeostasis.The Journal of nutritional biochemistry · 2015Review
- The double zinc finger domain and adjacent accessory domain from the transcription factor loss of zinc sensing 1 (loz1) are necessary for DNA binding and zinc sensing.The Journal of biological chemistry · 2014Article
- Peroxiredoxin chaperone activity is critical for protein homeostasis in zinc-deficient yeast.The Journal of biological chemistry · 2013Article
- Zinc finger protein Loz1 is required for zinc-responsive regulation of gene expression in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- Zinc-responsive coactivator recruitment by the yeast Zap1 transcription factor.MicrobiologyOpen · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies suggested that the zinc-responsive Zap1 transcriptional activator directly regulates the expression of over 80 genes in Saccharomyces cerevisiae. Many of these genes play key roles to enhance the ability of yeast cells to grow under zinc-limiting conditions. Zap1 is unusual among transcriptional activators in that it contains two activation domains, designated AD1 and AD2, which are regulated independently by zinc. These two domains are evolutionarily conserved among Zap1 orthologs suggesting that they are both important for Zap1 function. In this study, we have examined the roles of AD1 and AD2 in low zinc growth and the regulation of Zap1 target gene expression. Using alleles that are specifically disrupted for either AD1 or AD2 function, we found that these domains are not redundant, and both are important for normal growth in low zinc. AD1 plays the primary role in zinc-responsive gene regulation, whereas AD2 is required for maximal expression of only a few target promoters. AD1 alone is capable of driving full expression of most Zap1 target genes and dictates the kinetics of Zap1 gene induction in response to zinc withdrawal. Surprisingly, we found that AD1 is less active in zinc-limited cells under heat stress and AD2 plays a more important role under those conditions. These results suggest that AD2 may contribute more to Zap1 function when zinc deficiency is combined with other environmental stresses. In the course of these studies, we also found that the heat shock response is induced under conditions of severe zinc deficiency.
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