ArticleThe Journal of biological chemistry2011
Efficient and rapid nucleosome traversal by RNA polymerase II depends on a combination of transcript elongation factors.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence.Nature communications · 2024Article
- The long transcript of lncRNA TMPO-AS1 promotes bone metastases of prostate cancer by regulating the CSNK2A1/DDX3X complex in Wnt/β-catenin signaling.Cell death discovery · 2023Article
- Promoter-proximal nucleosomes attenuate RNA polymerase II transcription through TFIID.The Journal of biological chemistry · 2023Article
- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Molecular basis of transcriptional pausing, stalling, and transcription-coupled repair initiation.Biochimica et biophysica acta. Gene regulatory mechanisms · 2021Review
- Cockayne syndrome B protein acts as an ATP-dependent processivity factor that helps RNA polymerase II overcome nucleosome barriers.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RNA polymerase I (Pol I) passage through nucleosomes depends on Pol I subunits binding its lobe structure.The Journal of biological chemistry · 2020Article
- Promoter-proximal pausing of RNA polymerase II: a nexus of gene regulation.Genes & development · 2019Review
- TFS and Spt4/5 accelerate transcription through archaeal histone-based chromatin.Molecular microbiology · 2019Article
- Subtracting the sequence bias from partially digested MNase-seq data reveals a general contribution of TFIIS to nucleosome positioning.Epigenetics & chromatin · 2017Article
- The elongation factor Spt4/5 regulates RNA polymerase II transcription through the nucleosome.Nucleic acids research · 2017Article
- Pause & go: from the discovery of RNA polymerase pausing to its functional implications.Current opinion in cell biology · 2017Review
- Coupling of RNA Polymerase II Transcription Elongation with Pre-mRNA Splicing.Journal of molecular biology · 2016Review
- Nucleosomal Barrier to Transcription: Structural Determinants and Changes in Chromatin Structure.Biochemistry & molecular biology journal · 2016Article
- Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Molecular mechanisms of transcription through single-molecule experiments.Chemical reviews · 2014Review
- Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RNA polymerase II transcription elongation control.Chemical reviews · 2013Review
- Mechanism of transcription through a nucleosome by RNA polymerase II.Biochimica et biophysica acta · 2013Review
- Molecular traffic jams on DNA.Annual review of biophysics · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleosome is generally found to be a strong barrier to transcript elongation by RNA polymerase II (pol II) in vitro. The elongation factors TFIIF and TFIIS have been shown to cooperate in maintaining pol II in the catalytically competent state on pure DNA templates. We now show that although TFIIF or TFIIS alone is modestly stimulatory for nucleosome traversal, both factors together increase transcription through nucleosomes in a synergistic manner. We also studied the effect of TFIIF and TFIIS on transcription of nucleosomes containing a Sin mutant histone. The Sin point mutations reduce critical histone-DNA contacts near the center of the nucleosome. Significantly, we found that nucleosomes with a Sin mutant histone are traversed to the same extent and at nearly the same rate as equivalent pure DNA templates if both TFIIS and TFIIF are present. Thus, the nucleosome is not necessarily an insurmountable barrier to transcript elongation by pol II. If unfolding of template DNA from the nucleosome surface is facilitated and the tendency of pol II to retreat from barriers is countered, transcription of nucleosomal templates can be rapid and efficient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.