ArticleBMC microbiology2010
Rapid Leptospira identification by direct sequencing of the diagnostic PCR products in New Caledonia.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 2 of them syntheses that pooled it.
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Who cites it
50 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pooled it
- A systematic review of human and animal leptospirosis in the Pacific Islands reveals pathogen and reservoir diversity.PLoS neglected tropical diseases · 2018Pooled it
- Genetic Diversity ofPathogens (Basel, Switzerland) · 2026Article
- Leptospirosis Incidence at Four Sites in Sub-Saharan Africa and South East Asia: An International Multi-Site Hybrid Surveillance Study.Open forum infectious diseases · 2026Article
- Leptospirosis Prevalence and Risk Factors Among Patients Presenting With Fever to 4 Healthcare Sites in Sub-Saharan Africa and South East Asia: An International Multisite Observational and Nested Case-Control Study.The Journal of infectious diseases · 2026Observational
- Molecular detection of a Leptospira lineage with high similarity to Leptospira kirschneri in human, environmental, and rodent samples from Gilan Province, Iran: an integrated field and laboratory investigation.GMS hygiene and infection control · 2026Article
- Anti-inflammatory cytokine profile and Jarisch-Herxheimer reaction in Leptospirosis patients: A prospective case-series study in New Caledonia.PLoS neglected tropical diseases · 2025Observational
- Leptospira borgptersenii and Leptospira interrogans identified in wild mammals in Rio Grande do Sul, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- First isolation and genotyping of pathogenic Leptospira spp. from Austria.Scientific reports · 2024Article
- Molecular Characterization ofPathogens (Basel, Switzerland) · 2023Article
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.BMC infectious diseases · 2023Article
- Utility evaluation of two molecular methods forHeliyon · 2023Article
- Genetic diversity ofFrontiers in cellular and infection microbiology · 2023Article
- Diversity of Leptospira spp. in bats and rodents from Papua New Guinea.Transboundary and emerging diseases · 2022Article
- Comparison of the Serion IgM ELISA and Microscopic Agglutination Test for diagnosis of Leptospira spp. infections in sera from different geographical origins and estimation of Leptospira seroprevalence in the Wiwa indigenous population from Colombia.PLoS neglected tropical diseases · 2022Article
- Article
- Identification of Leptospira spp. in the animal-environment interface (swine-water) in pig production cycle.Tropical animal health and production · 2021Article
- Evaluation of three qPCR for the detection of pathogenic leptospires in domestic animals in NicaraguaBiomedica : revista del Instituto Nacional de Salud · 2020Article
- Circulating genotypes of Leptospira in French Polynesia : An 9-year molecular epidemiology surveillance follow-up study.PLoS neglected tropical diseases · 2020Article
- Laboratory Diagnosis of Bovine Abortions Caused by Non-Maintenance PathogenicPathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost of the current knowledge of leptospirosis epidemiology originates from serological results obtained with the reference Microscopic Agglutination Test (MAT). However, inconsistencies and weaknesses of this diagnostic technique are evident. A growing use of PCR has improved the early diagnosis of leptospirosis but a drawback is that it cannot provide information on the infecting Leptospira strain which provides important epidemiologic data. Our work is aimed at evaluating if the sequence polymorphism of diagnostic PCR products could be used to identify the infecting Leptospira strains in the New Caledonian environment.
resultsBoth the lfb1 and secY diagnostic PCR products displayed a sequence polymorphism that could prove useful in presumptively identifying the infecting leptospire. Using both this polymorphism and MLST results with New Caledonian isolates and clinical samples, we confirmed the epidemiological relevance of the sequence-based identification of Leptospira strains. Additionally, we identified one cluster of L. interrogans that contained no reference strain and one cluster of L. borgpetersenii found only in the introduced Rusa deer Cervus timorensis russa that is its probable reservoir.
conclusionsThe sequence polymorphism of diagnostic PCR products proved useful in presumptively identifying the infecting Leptospira strains. This could contribute to a better understanding of leptospirosis epidemiology by providing epidemiological information that cannot be directly attained from the use of PCR as an early diagnostic test for leptospirosis.
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