ArticleBMC molecular biology2010
The CTCF insulator protein forms an unusual DNA structure.
Article in BMC molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Assessing base-resolution DNA mechanics on the genome scale.Nucleic acids research · 2023Article
- Contribution of the IGCR1 regulatory element and the 3'Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The role of chromatin loop extrusion in antibody diversification.Nature reviews. Immunology · 2022Review
- CTCF as a boundary factor for cohesin-mediated loop extrusion: evidence for a multi-step mechanism.Nucleus (Austin, Tex.) · 2020Review
- Molecular basis of CTCF binding polarity in genome folding.Nature communications · 2020Article
- Article
- CTCF mediates chromatin looping via N-terminal domain-dependent cohesin retention.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- One protein to rule them all: The role of CCCTC-binding factor in shaping human genome in health and disease.Seminars in cell & developmental biology · 2019Review
- Vitamin D Deficiency: Effects on Oxidative Stress, Epigenetics, Gene Regulation, and Aging.Biology · 2019Review
- CTCF-Induced Circular DNA Complexes Observed by Atomic Force Microscopy.Journal of molecular biology · 2018Article
- Pan-cancer screen for mutations in non-coding elements with conservation and cancer specificity reveals correlations with expression and survival.NPJ genomic medicine · 2018Article
- Recent evidence that TADs and chromatin loops are dynamic structures.Nucleus (Austin, Tex.) · 2018Review
- Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites.Cell research · 2017Article
- Article
- CTCF: making the right connections.Genes & development · 2016Review
- A CTCF Code for 3D Genome Architecture.Cell · 2015Article
- Epigenetics and memigenetics.Cellular and molecular life sciences : CMLS · 2014Review
- CTCF regulates Kaposi's sarcoma-associated herpesvirus latency transcription by nucleosome displacement and RNA polymerase programming.Journal of virology · 2013Article
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe CTCF insulator protein is a highly conserved zinc finger protein that has been implicated in many aspects of gene regulation and nuclear organization. The protein has been hypothesized to organize the human genome by forming DNA loops.
resultsIn this paper, we report biochemical evidence to support the role for CTCF in forming DNA loops. We have measured DNA bending by CTCF at the chicken HS4 β-globin FII insulator element in vitro and have observed a unique DNA structure with aberrant electrophoretic mobility which we believe to be a DNA loop. CTCF is able to form this unusual DNA structure at two other binding sites: the c-myc P2 promoter and the chicken F1 lysozyme gene silencer. We also demonstrate that the length though not the sequence of the DNA downstream of the binding site is important for the ability of CTCF to form this unusual DNA structure. We hypothesize that a single CTCF protein molecule is able to act as a "looper" possibly through the use of several of its zinc fingers.
conclusionsCTCF is able to form an unusual DNA structure through the zinc finger domain of the protein. This unusual DNA structure is formed in a directional manner by the CTCF protein. The findings described in this paper suggest mechanisms by which CTCF is able to form DNA loops, organize the mammalian genome and function as an insulator protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.