ArticleThe Biochemical journal2011
Crystal structure of unliganded TRAP: implications for dynamic allostery.
Article in The Biochemical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Designed, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH-Dependent Assembly.Macromolecular rapid communications · 2025Article
- Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Insights into Ligand-Mediated Activation of an Oligomeric Ring-Shaped Gene-Regulatory Protein from Solution- and Solid-State NMR.Journal of molecular biology · 2024Article
- Insights into Ligand-Mediated Activation of an Oligomeric Ring-Shaped Gene-Regulatory Protein from Solution- and Solid-State NMR.bioRxiv : the preprint server for biology · 2024Article
- Structural basis of nearest-neighbor cooperativity in the ring-shaped gene regulatory protein TRAP from protein engineering and cryo-EM.bioRxiv : the preprint server for biology · 2024Article
- Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change.ACS nanoscience Au · 2022Article
- Design of Polypeptides Self-Assembling into Antifouling Coatings: Exploiting Multivalency.Biomacromolecules · 2022Article
- Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles.Nano letters · 2022Article
- Artificial Protein Cage Delivers Active Protein Cargos to the Cell Interior.Biomacromolecules · 2021Article
- Allosteric Activation of PI3Kα Results in Dynamic Access to Catalytically Competent Conformations.Structure (London, England : 1993) · 2020Article
- Article
- Mechanistic Models Fit to Variable Temperature Calorimetric Data Provide Insights into Cooperativity.Biophysical journal · 2017Article
- Homotropic cooperativity from the activation pathway of the allosteric ligand-responsive regulatory trp RNA-binding attenuation protein.Biochemistry · 2013Article
- Mechanisms of allosteric gene regulation by NMR quantification of microsecond-millisecond protein dynamics.Journal of molecular biology · 2012Article
- Trp RNA-binding attenuation protein: modifying symmetry and stability of a circular oligomer.PloS one · 2012Article
- Theory and simulation of diffusion-influenced, stochastically gated ligand binding to buried sites.The Journal of chemical physics · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Allostery is vital to the function of many proteins. In some cases, rather than a direct steric effect, mutual modulation of ligand binding at spatially separated sites may be achieved through a change in protein dynamics. Thus changes in vibrational modes of the protein, rather than conformational changes, allow different ligand sites to communicate. Evidence for such an effect has been found in TRAP (trp RNA-binding attenuation protein), a regulatory protein found in species of Bacillus. TRAP is part of a feedback system to modulate expression of the trp operon, which carries genes involved in tryptophan synthesis. Negative feedback is thought to depend on binding of tryptophan-bound, but not unbound, TRAP to a specific mRNA leader sequence. We find that, contrary to expectations, at low temperatures TRAP is able to bind RNA in the absence of tryptophan, and that this effect is particularly strong in the case of Bacillus stearothermophilus TRAP. We have solved the crystal structure of this protein with no tryptophan bound, and find that much of the structure shows little deviation from the tryptophan-bound form. These data support the idea that tryptophan may exert its effect on RNA binding by TRAP through dynamic and not structural changes, and that tryptophan binding may be mimicked by low temperature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.