ArticleTissue engineering. Part C, Methods2011
Simultaneous application of interstitial flow and cyclic mechanical strain to a three-dimensional cell-seeded hydrogel.
Article in Tissue engineering. Part C, Methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Soft bioreactor systems: a necessary step toward engineered MSK soft tissue?Frontiers in robotics and AI · 2024Review
- Cells function as a ternary logic gate to decide migration direction under integrated chemical and fluidic cues.Lab on a chip · 2023Article
- Transcriptomic analysis of a 3D blood-brain barrier model exposed to disturbed fluid flow.Fluids and barriers of the CNS · 2022Article
- A new insight into a thermoplastic microfluidic device aimed at improvement of oxygenation process and avoidance of shear stress during cell culture.Biomedical microdevices · 2022Article
- Engineering Biomaterials and Approaches for Mechanical Stretching of Cells in Three Dimensions.Frontiers in bioengineering and biotechnology · 2020Review
- Spatiotemporal delivery of basic fibroblast growth factor to directly and simultaneously attenuate cardiac fibrosis and promote cardiac tissue vascularization following myocardial infarction.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
- Injection of mesenchymal stromal cells into a mechanically stimulated in vitro model of cardiac fibrosis has paracrine effects on resident fibroblasts.Cytotherapy · 2014Article
- A poroelastic model describing nutrient transport and cell stresses within a cyclically strained collagen hydrogel.Biophysical journal · 2013Article
- Microfluidics embedded within extracellular matrix to define vascular architectures and pattern diffusive gradients.Lab on a chip · 2013Article
- Mechanostimulation protocols for cardiac tissue engineering.BioMed research international · 2013Review
- Hydrogel-based nanocomposites and mesenchymal stem cells: a promising synergistic strategy for neurodegenerative disorders therapy.TheScientificWorldJournal · 2013Review
- Finite-element modeling of viscoelastic cells during high-frequency cyclic strain.Journal of functional biomaterials · 2012Article
- Effects of convective transport on chemical signal propagation in epithelia.Biophysical journal · 2012Article
- Interstitial fluid flow and cyclic strain differentially regulate cardiac fibroblast activation via AT1R and TGF-β1.Experimental cell research · 2012Article
- A linear, biphasic model incorporating a brinkman term to describe the mechanics of cell-seeded collagen hydrogels.Annals of biomedical engineering · 2011Article
Corrections and comments
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2 authors.
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Abstract
The present study describes the design and validation of a simple apparatus to apply simultaneous mechanical and fluidic stress to three-dimensional (3D) cell-seeded collagen hydrogels. Constructs were formed in wells in a silicone substrate that could be stretched cyclically, and were also fitted with inlet ports to apply fluid flow. Acid etching was used to retain adhesion of the gels to the walls of the well, and an acellular layer of collagen hydrogel was used to distribute flow evenly. Finite element modeling showed that 5% uniaxial strain applied to the entire silicone substrate resulted in ∼6.5% strain in each of the gel constructs. Permeability testing and flow observation showed that acellular hydrogels were fourfold more permeable than cardiac fibroblast-seeded gels, and that the fluid distributed evenly in the acellular layer before entering the cell-seeded gel. Viability testing and imaging demonstrated that cells remained viable with expected fibroblast morphology for the 120 h duration of the experiments. These results demonstrate that this simple bioreactor can be used to study the effects of mechanical strain and interstitial flow in 3D protein hydrogels. Such 3D tissue models have utility in studying cell and tissue responses to their mechanical environment.
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