ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2011
In vitro culture of human fetal corneal endothelial cells.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- The Preservation and Reuse of Lenticules Extracted via Small Incision Lenticule Extraction (SMILE): A Narrative Review.Bioengineering (Basel, Switzerland) · 2025Review
- Constructing a Novel Three-Dimensional Biomimetic Corneal Endothelium Graft by Culturing Corneal Endothelium Cells on Compressed Collagen Gels.Chinese medical journal · 2018Article
- Corneal lenticule storage before reimplantation.Molecular vision · 2017Article
- 3D map of the human corneal endothelial cell.Scientific reports · 2016Article
- Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium.Journal of functional biomaterials · 2015Review
- The effects of ROCK inhibitor Y-27632 on injectable spheroids of bovine corneal endothelial cells.Cellular reprogramming · 2015Article
- In vitro expansion of corneal endothelial cells on biomimetic substrates.Scientific reports · 2015Article
- Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.Human molecular genetics · 2013Article
- Regulation of functional corneal endothelial cells isolated from sphere colonies by Rho-associated protein kinase inhibitor.Experimental and therapeutic medicine · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo explore and optimize a proper culture system for human fetal corneal endothelial cells (hFCECs), including the methods of primary culture, passage and cryopreservation.
methodsFresh fetal corneas were explanted to propagate primary corneal endothelial cells. The cells were cultured in DMEM/F-12 supplemented with 10% fetal bovine serum (FBS) in the absence or presence of the extracts from bovine corneal endothelium cells (bCECs), and the cells were identified with immunocytochemical staining. The passage and cryopreservation of hFCECs were optimized according to previous reports on adult corneal endothelial cells.
resultsUsing the explant culture method, hFCECs migrated successfully within 3 days and assumed polygonal-shaped corneal endothelial morphology. The optimizing methods were 0.125% trypsin + 0.02% EDTA for passage and 10% DMSO + 90% FBS for cryopreservation. Recovered hFCECs from cryopreservation remained typical morphology and immunological markers of corneal endothelial cells, including positive staining of NSE, Nestin, Ki67 and ZO-1, and negative staining of CK3/12, which demonstrated that they retained the characterizations of corneal endothelial cells and proliferative capacity. Moreover, the extracts from bCECs can promote the proliferative capacity of hFCECs significantly, while maintaining their typical endothelial morphology.
conclusionsThe culture conditions of human fetal corneal endothelial cells were firstly optimized, including the primary culture, passage and cryopreservation. Meanwhile, we confirmed that the extracts from bovine corneal endothelium promoted the proliferative capacity while maintaining the morphology of hFCECs in vitro.
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