ArticleProceedings of the National Academy of Sciences of the United States of America2011
Intracellular organelles mediate cytoplasmic pulling force for centrosome centration in the Caenorhabditis elegans early embryo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 154 citations in OpenAlex.
- Microtubule pushing and cortical pulling cooperate to orient and position the mitotic spindle.The EMBO journal · 2026Article
- Divergence of Cortical Force-Generating Mechanisms Underlies Differences in Spindle Behavior betweenbioRxiv : the preprint server for biology · 2026Article
- Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.PLoS genetics · 2026Article
- Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transport.iScience · 2025Article
- Asymmetry in centrosome maturation revealed through AIR-1 dynamics in the early Caenorhabditis elegans embryo.Scientific reports · 2025Article
- ZYG-12/Hook's dual role as a dynein adaptor for early endosomes and nuclei is regulated by alternative splicing of its cargo binding domain.Molecular biology of the cell · 2025Article
- Cytoplasmic flow is a cell size sensor that scales anaphase.Nature cell biology · 2025Article
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A cytokinetic ring-driven cell rotation achieves Hertwig's rule in early development.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in thebioRxiv : the preprint server for biology · 2024Article
- Enucleation of theLife science alliance · 2024Article
- Dynein localization and pronuclear movement in the C. elegans zygote.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Nuclear speed and cycle length co-vary with local density during syncytial blastoderm formation in a cricket.Nature communications · 2022Article
- Review
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Roadmap for the multiscale coupling of biochemical and mechanical signals during development.Physical biology · 2021Review
13 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The centrosome is generally maintained at the center of the cell. In animal cells, centrosome centration is powered by the pulling force of microtubules, which is dependent on cytoplasmic dynein. However, it is unclear how dynein brings the centrosome to the cell center, i.e., which structure inside the cell functions as a substrate to anchor dynein. Here, we provide evidence that a population of dynein, which is located on intracellular organelles and is responsible for organelle transport toward the centrosome, generates the force required for centrosome centration in Caenorhabditis elegans embryos. By using the database of full-genome RNAi in C. elegans, we identified dyrb-1, a dynein light chain subunit, as a potential subunit involved in dynein anchoring for centrosome centration. DYRB-1 is required for organelle movement toward the minus end of the microtubules. The temporal correlation between centrosome centration and the net movement of organelle transport was found to be significant. Centrosome centration was impaired when Rab7 and RILP, which mediate the association between organelles and dynein in mammalian cells, were knocked down. These results indicate that minus end-directed transport of intracellular organelles along the microtubules is required for centrosome centration in C. elegans embryos. On the basis of this finding, we propose a model in which the reaction forces of organelle transport generated along microtubules act as a driving force that pulls the centrosomes toward the cell center. This is the first model, to our knowledge, providing a mechanical basis for cytoplasmic pulling force for centrosome centration.
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