ArticleAntimicrobial agents and chemotherapy2011
Characterization of the biosynthesis gene cluster for the pyrrole polyether antibiotic calcimycin (A23187) in Streptomyces chartreusis NRRL 3882.
Article in Antimicrobial agents and chemotherapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 73 citations in OpenAlex.
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- Exploring the Role of a Putative Secondary Metabolite Biosynthesis Pathway inMicroorganisms · 2024Article
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- Precezomycin, a novel antibiotic biosynthetic precursor of cezomycin, from actinomycete Kitasatospora putterlickiae 10-13.The Journal of antibiotics · 2024Article
- Non-canonical two-step biosynthesis of anti-oomycete indole alkaloids in Kickxellales.Fungal biology and biotechnology · 2023Article
- Biosynthesis-Guided Discovery and Engineering of α-Pyrone Natural Products from Type I Polyketide Synthases.ACS chemical biology · 2023Article
- C-N bond formation by a polyketide synthase.Nature communications · 2023Article
- Genomics-Driven Discovery of Benzoxazole Alkaloids from the Marine-DerivedMolecules (Basel, Switzerland) · 2023Article
- Recent developments in the identification and biosynthesis of antitumor drugs derived from microorganisms.Engineering microbiology · 2022Review
- Effects of 4-Br-A23187 on Bacillus subtilis cells and unilamellar vesicles reveal it to be a potent copper ionophore.Proteomics · 2022Article
- Alternative Benzoxazole Assembly Discovered in Anaerobic Bacteria Provides Access to Privileged Heterocyclic Scaffold.Angewandte Chemie (International ed. in English) · 2022Article
- Influence of Amino Acid Feeding on Production of Calcimycin and Analogs inInternational journal of environmental research and public health · 2021Article
- Construction of an Alternative NADAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- The Biosynthesis of the Benzoxazole in Nataxazole Proceeds via an Unstable Ester and has Synthetic Utility.Angewandte Chemie (International ed. in English) · 2020Article
- Phylogenetic Studies on the Prodigiosin Biosynthetic Operon.Current microbiology · 2019Article
- The biosynthetic pathway to ossamycin, a macrocyclic polyketide bearing a spiroacetal moiety.PloS one · 2019Article
- Computational identification of co-evolving multi-gene modules in microbial biosynthetic gene clusters.Communications biology · 2019Article
- Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882.Applied and environmental microbiology · 2018Article
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7 authors at 1 institution in 1 country.
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Abstract
The pyrrole polyether antibiotic calcimycin (A23187) is a rare ionophore that is specific for divalent cations. It is widely used as a biochemical and pharmacological tool because of its multiple, unique biological effects. Here we report on the cloning, sequencing, and mutational analysis of the 64-kb biosynthetic gene cluster from Streptomyces chartreusis NRRL 3882. Gene replacements confirmed the identity of the gene cluster, and in silico analysis of the DNA sequence revealed 27 potential genes, including 3 genes for the biosynthesis of the α-ketopyrrole moiety, 5 genes that encode modular type I polyketide synthases for the biosynthesis of the spiroketal ring, 4 genes for the biosynthesis of 3-hydroxyanthranilic acid, an N-methyltransferase tailoring gene, a resistance gene, a type II thioesterase gene, 3 regulatory genes, 4 genes with other functions, and 5 genes of unknown function. We propose a pathway for the biosynthesis of calcimycin and assign the genes to the biosynthesis steps. Our findings set the stage for producing much desired calcimycin derivatives using genetic modification instead of chemical synthesis.
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