ArticleMolecular cell2010
Recruitment timing and dynamics of transcription factors at the Hsp70 loci in living cells.
Article in Molecular cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Article
- Regulation of RNA polymerase II transcription through re-initiation and bursting.Molecular cell · 2025Review
- Feeling the force from within - new tools and insights into nuclear mechanotransduction.Journal of cell science · 2025Review
- The role of transcription bodies in gene expression: what embryos teach us.Biochemical Society transactions · 2025Review
- Live-cell imaging of RNA Pol II and elongation factors distinguishes competing mechanisms of transcription regulation.Molecular cell · 2024Article
- Aromatic and arginine content drives multiphasic condensation of protein-RNA mixtures.Biophysical journal · 2024Article
- A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis.Nature communications · 2024Article
- Pri peptides temporally coordinate transcriptional programs during epidermal differentiation.Science advances · 2024Article
- ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes.Nature communications · 2023Article
- Insights into Spt6: a histone chaperone that functions in transcription, DNA replication, and genome stability.Trends in genetics : TIG · 2023Review
- Spatial regulation of Drosophila ovarian Follicle Stem Cell division rates and cell cycle transitions.PLoS genetics · 2023Article
- The NELF pausing checkpoint mediates the functional divergence of Cdk9.Nature communications · 2023Article
- Thermodynamic origins of two-component multiphase condensates of proteins.Chemical science · 2023Article
- Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Poly(ADP-ribose) in Condensates: The PARtnership of Phase Separation and Site-Specific Interactions.International journal of molecular sciences · 2022Review
- A sePARate phase? Poly(ADP-ribose) versus RNA in the organization of biomolecular condensates.Nucleic acids research · 2022Review
- A transcriptional cycling model recapitulates chromatin-dependent features of noisy inducible transcription.PLoS computational biology · 2022Article
- Phase separation of RNA-binding protein promotes polymerase binding and transcription.Nature chemical biology · 2022Article
- Transcription Factor Dynamics.Cold Spring Harbor perspectives in biology · 2021Review
- Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.Molecular cancer · 2021Article
29 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
Abstract
Chromatin immunoprecipitation (ChIP) studies provide snapshots of factors on chromatin in cell populations. Here, we use live-cell imaging to examine at high temporal resolution the recruitment and dynamics of transcription factors to the inducible Hsp70 loci in individual Drosophila salivary gland nuclei. Recruitment of the master regulator, HSF, is first detected within 20 s of gene activation; the timing of its recruitment resolves from RNA polymerase II and P-TEFb, and these factors resolve from Spt6 and Topo I. Remarkably, the recruitment of each factor is highly synchronous between different cells. In addition, fluorescence recovery after photobleaching (FRAP) analyses show that the entry and exit of multiple factors are progressively constrained upon gene activation, suggesting the gradual formation of a transcription compartment. Furthermore, we demonstrate that poly(ADP-ribose) (PAR) polymerase activity is required to maintain the transcription compartment. We propose that PAR polymers locally retain factors in a transcription compartment.
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