ArticleNeuron2010
Vesicular ATPase inserted into the plasma membrane of motor terminals by exocytosis alkalinizes cytosolic pH and facilitates endocytosis.
Article in Neuron, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 69 citations in OpenAlex.
- Pathological Interplay between Inflammation and Mitochondria Aggravates Glutamate Toxicity.International journal of molecular sciences · 2024Review
- Activation of TRPV1 Channels Inhibits the Release of Acetylcholine and Improves Muscle Contractility in Mice.Cellular and molecular neurobiology · 2023Article
- Postsynaptic Calcium Extrusion at the Mouse Neuromuscular Junction Alkalinizes the Synaptic Cleft.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Neuronal activity-induced, equilibrative nucleoside transporter-dependent, somatodendritic adenosine release revealed by a GRAB sensor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Quantitative Synaptic Biology: A Perspective on Techniques, Numbers and Expectations.International journal of molecular sciences · 2020Review
- Interstitial ions: A key regulator of state-dependent neural activity?Progress in neurobiology · 2020Review
- Activity and Cytosolic NaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Chromophore-Assisted Light Inactivation of the V-ATPase V0c Subunit Inhibits Neurotransmitter Release Downstream of Synaptic Vesicle Acidification.Molecular neurobiology · 2019Article
- Expression of plasma membrane calcium ATPases confers CaScientific reports · 2019Article
- Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions.Journal of visualized experiments : JoVE · 2018Article
- A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry.Nature communications · 2018Article
- VGLUT1 functions as a glutamate/proton exchanger with chloride channel activity in hippocampal glutamatergic synapses.Nature communications · 2017Article
- Diverse modes of synaptic signaling, regulation, and plasticity distinguish two classes ofeLife · 2017Article
- Fatty acid synthase cooperates with protrudin to facilitate membrane outgrowth of cellular protrusions.Scientific reports · 2017Article
- Distinct Actions of Voltage-Activated CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- VGLUT soothes the sour synapse.The Journal of physiology · 2017Article
- NaThe Journal of physiology · 2017Article
- Acetazolamide potentiates the afferent drive to prefrontal cortex in vivo.Physiological reports · 2017Article
- Sources of protons and a role for bicarbonate in inhibitory feedback from horizontal cells to cones in Ambystoma tigrinum retina.The Journal of physiology · 2016Article
- Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS.International journal of molecular sciences · 2016Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Key components of vesicular neurotransmitter release, such as Ca(2+) influx and membrane recycling, are affected by cytosolic pH. We measured the pH-sensitive fluorescence of Yellow Fluorescent Protein transgenically expressed in mouse motor nerve terminals, and report that Ca(2+) influx elicited by action potential trains (12.5-100 Hz) evokes a biphasic pH change: a brief acidification (∼ 13 nM average peak increase in [H(+)]), followed by a prolonged alkalinization (∼ 30 nM peak decrease in [H(+)]) that outlasts the stimulation train. The alkalinization is selectively eliminated by blocking vesicular exocytosis with botulinum neurotoxins, and is prolonged by the endocytosis-inhibitor dynasore. Blocking H(+) pumping by vesicular H(+)-ATPase (with folimycin or bafilomycin) suppresses stimulation-induced alkalinization and reduces endocytotic uptake of FM1-43. These results suggest that H(+)-ATPase, known to transfer cytosolic H(+) into prefused vesicles, continues to extrude cytosolic H(+) after being exocytotically incorporated into the plasma membrane. The resulting cytosolic alkalinization may facilitate vesicular endocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.