ArticleRetrovirology2010
Mouse DNA contamination in human tissue tested for XMRV.
Article in Retrovirology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 114 citations in OpenAlex.
- MMTV-like Viruses and Human Breast Cancer: Evidence for Causality.Current issues in molecular biology · 2026Review
- Human sporadic breast carcinoma histotypes driven by the Human Betaretrovirus homologous to Mouse Mammary Tumor Virus.International journal of cancer · 2025Article
- Presence of a mouse mammary tumour virus-like in feline lymphomas: a preliminary study.Infectious agents and cancer · 2022Review
- Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas.Animals : an open access journal from MDPI · 2021Article
- Article
- Article
- A trip down memory lane with Retrovirology.Retrovirology · 2019Article
- Article
- Xenotropic Murine Leukemia Virus-Related Virus (XMRV) and the Safety of the Blood Supply.Clinical microbiology reviews · 2016Review
- Microbial Community Responses to Increased Water and Organic Matter in the Arid Soils of the McMurdo Dry Valleys, Antarctica.Frontiers in microbiology · 2016Article
- Article
- Lack of evidence for retroviral infections formerly related to chronic fatigue in Spanish fibromyalgia patients.Virology journal · 2013Article
- Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.Journal of virology · 2013Article
- No evidence of xenotropic murine leukemia virus-related virus transmission by blood transfusion from infected rhesus macaques.Journal of virology · 2013Article
- No detection of the retrovirus xenotropic murine leukemia virus-related virus in individuals with hemophilia.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2013Article
- No association between XMRV or related gammaretroviruses in Australian prostate cancer patients.Virology journal · 2013Article
- Infection of Xenotransplanted Human Cell Lines by Murine Retroviruses: A Lesson Brought Back to Light by XMRV.Frontiers in oncology · 2013Article
- Open access, moving to the fore.Retrovirology · 2012Article
- Recombinant origin, contamination, and de-discovery of XMRV.Current opinion in virology · 2012Review
- XMRV accelerates cellular proliferation, transformational activity, and invasiveness of prostate cancer cells by downregulating p27(Kip1).The Prostate · 2012Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 4 countries.
Funding
Abstract
backgroundWe used a PCR-based approach to study the prevalence of genetic sequences related to a gammaretrovirus, xenotropic murine leukemia virus-related virus, XMRV, in human prostate cancer. This virus has been identified in the US in prostate cancer patients and in those with chronic fatigue syndrome. However, with the exception of two patients in Germany, XMRV has not been identified in prostate cancer tissue in Europe. Most putative associations of new or old human retroviruses with diseases have turned out to be due to contamination. We have looked for XMRV sequences in DNA extracted from formalin-fixed paraffin- embedded prostate tissues. To control for contamination, PCR assays to detect either mouse mitochondrial DNA (mtDNA) or intracisternal A particle (IAP) long terminal repeat DNA were run on all samples, owing to their very high copy number in mouse cells.
resultsIn general agreement with the US prevalence, XMRV-like sequences were found in 4.8% of prostate cancers. However, these were also positive, as were 21.5% of XMRV-negative cases, for IAP sequences, and many, but not all were positive for mtDNA sequences.
conclusionsThese results show that contamination with mouse DNA is widespread and detectable by the highly sensitive IAP assay, but not always with less sensitive assays, such as murine mtDNA PCR. This study highlights the ubiquitous presence of mouse DNA in laboratory specimens and offers a means of rigorous validation for future studies of murine retroviruses in human disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.