ArticleJournal of the American Chemical Society2011
Metal-substituted protein MRI contrast agents engineered for enhanced relaxivity and ligand sensitivity.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Liposomal nanoprobes actuated by engineered water channels for sensitive detection of molecular targets by MRI.Nature biomedical engineering · 2026Article
- High-Throughput Screening of Co-Protoporphyrin IX-Binding Proteins for Enhanced Hydrogen Production.Molecules (Basel, Switzerland) · 2026Article
- Provision of a Redox Donor to Oxy-Ferrous PrnB Is Required for Pyrrolnitrin Synthesis.Biochemistry · 2025Article
- Regulation of ferryl reactivity by the cytochrome P450 decarboxylase OleT.Journal of inorganic biochemistry · 2025Article
- Directed evolution ofChemical science · 2024Article
- Biogenic Imaging Contrast Agents.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Longitudinal manganese-enhanced magnetic resonance imaging of neural projections and activity.NMR in biomedicine · 2022Review
- Corrole-protein interactions in H-NOX and HasA.RSC chemical biology · 2022Article
- Directed Evolution of Artificial Metalloenzymes in Whole Cells.Angewandte Chemie (International ed. in English) · 2022Article
- Assembly and Evolution of Artificial Metalloenzymes withinJournal of the American Chemical Society · 2022Article
- Designer Heme Proteins: Achieving Novel Function with Abiological Heme Analogues.Accounts of chemical research · 2021Article
- De novo biosynthesis of a nonnatural cobalt porphyrin cofactor inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Highly malleable haem-binding site of the haemoprotein HasA permits stable accommodation of bulky tetraphenylporphycenes.RSC advances · 2019Article
- Noble-Metal Substitution in Hemoproteins: An Emerging Strategy for Abiological Catalysis.Accounts of chemical research · 2019Article
- Endogenous insertion of non-native metalloporphyrins into human membrane cytochrome P450 enzymes.The Journal of biological chemistry · 2018Article
- Insulin Hexamer-Caged Gadolinium Ion as MRI Contrast-o-phore.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Orthogonal Expression of an Artificial Metalloenzyme for Abiotic Catalysis.Chembiochem : a European journal of chemical biology · 2017Article
- Biomolecular MRI reporters: Evolution of new mechanisms.Progress in nuclear magnetic resonance spectroscopy · 2017Review
- Metal Substitution Modulates the Reactivity and Extends the Reaction Scope of Myoglobin Carbene Transfer Catalysts.Advanced synthesis & catalysis · 2017Article
- Abiological catalysis by artificial haem proteins containing noble metals in place of iron.Nature · 2016Article
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Authors and funding
4 authors.
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Abstract
Engineered metalloproteins constitute a flexible new class of analyte-sensitive molecular imaging agents detectable by magnetic resonance imaging (MRI), but their contrast effects are generally weaker than synthetic agents. To augment the proton relaxivity of agents derived from the heme domain of cytochrome P450 BM3 (BM3h), we formed manganese(III)-containing proteins that have higher electron spin than their native ferric iron counterparts. Metal substitution was achieved by coexpressing BM3h variants with the bacterial heme transporter ChuA in Escherichia coli and supplementing the growth medium with Mn3+-protoporphyrin IX. Manganic BM3h variants exhibited up to 2.6-fold higher T1 relaxivities relative to native BM3h at 4.7 T. Application of ChuA-mediated porphyrin substitution to a collection of thermostable chimeric P450 domains resulted in a stable, high-relaxivity BM3h derivative displaying a 63% relaxivity change upon binding of arachidonic acid, a natural ligand for the P450 enzyme and an important component of biological signaling pathways. This work demonstrates that protein-based MRI sensors with robust ligand sensitivity may be created with ease by including metal substitution among the toolkit of methods available to the protein engineer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.