ArticlePloS one2010
DNA aptamers as molecular probes for colorectal cancer study.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Covalent aptamers: agents with promising therapeutic and diagnostic potential.RSC chemical biology · 2026Review
- Aptamers as target-specific recognition elements in drug delivery.Advanced drug delivery reviews · 2025Review
- L-Histidine Modulates the Catalytic Activity and Conformational Changes of the HD3 Deoxyribozyme.Genes · 2024Article
- Bridging biological samples to functional nucleic acid biosensor applications: current enzymatic-based strategies for single-stranded DNA generation.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Review
- A novel DNA aptamer targeting lung cancer stem cells exerts a therapeutic effect by binding and neutralizing Annexin A2.Molecular therapy. Nucleic acids · 2022Article
- Review
- An automated microfluidic system for selection of aptamer probes against ovarian cancer tissues.Biomicrofluidics · 2019Article
- Microfluidic platforms for rapid screening of cancer affinity reagents by using tissue samples.Biomicrofluidics · 2018Article
- Aptamers: novelty tools for cancer biology.Oncotarget · 2018Review
- Aptamer Cell-Based Selection: Overview and Advances.Biomedicines · 2017Review
- Review
- Development of Cell-SELEX Technology and Its Application in Cancer Diagnosis and Therapy.International journal of molecular sciences · 2016Review
- An Optically-Transparent Aptamer-Based Detection System for Colon Cancer Applications Using Gold Nanoparticles Electrodeposited on Indium Tin Oxide.Sensors (Basel, Switzerland) · 2016Article
- Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells.Methods (San Diego, Calif.) · 2016Review
- Using modified aptamers for site specific protein-aptamer conjugations.Chemical science · 2016Article
- Identification of an aptamer through whole cell-SELEX for targeting high metastatic liver cancers.Oncotarget · 2016Article
- Generating Cell Targeting Aptamers for Nanotheranostics Using Cell-SELEX.Theranostics · 2016Review
- The Effects of SELEX Conditions on the Resultant Aptamer Pools in the Selection of Aptamers Binding to Bacterial Cells.Journal of molecular evolution · 2015Article
- An Apta-Biosensor for Colon Cancer Diagnostics.Sensors (Basel, Switzerland) · 2015Article
- Development of RNA aptamers as molecular probes for HER2(+) breast cancer study using cell-SELEX.Iranian journal of basic medical sciences · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundUnderstanding the molecular features of specific tumors can increase our knowledge about the mechanism(s) underlying disease development and progression. This is particularly significant for colorectal cancer, which is a heterogeneous complex of diseases developed in a sequential manner through a multistep carcinogenic process. As such, it is likely that tumors with similar characteristics might originate in the same manner and have a similar molecular behavior. Therefore, specific mapping of the molecular features can be potentially useful for both tumor classification and the development of appropriate therapeutic regimens. However, this can only be accomplished by developing high-affinity molecular probes with the ability to recognize specific markers associated with different tumors. Aptamers can most easily meet this challenge based on their target diversity, flexible manipulation and ease of development. METHODOLOGY AND
resultsUsing a method known as cell-based Systematic Evolution of Ligands by Exponential enrichment (cell-SELEX) and colorectal cancer cultured cell lines DLD-1 and HCT 116, we selected a panel of target-specific aptamers. Binding studies by flow cytometry and confocal microscopy showed that these aptamers have high affinity and selectivity. Our data further show that these aptamers neither recognize normal colon cells (cultured and fresh), nor do they recognize most other cancer cell lines tested. CONCLUSION/SIGNIFICANCE: The selected aptamers can identify specific biomarkers associated with colorectal cancers. We believe that these probes could be further developed for early disease detection, as well as prognostic markers, of colorectal cancers.
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