ArticlePloS one2010
Genome-wide screen for Mycobacterium tuberculosis genes that regulate host immunity.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Review
- Pathogenicity and virulence ofVirulence · 2023Review
- Article
- TheInternational journal of molecular sciences · 2022Review
- Extracellular Vesicles in Mycobacteria and Tuberculosis.Frontiers in cellular and infection microbiology · 2022Review
- Article
- Mycobacterium tuberculosis Rv2700 Contributes to Cell Envelope Integrity and Virulence.Journal of bacteriology · 2019Article
- Elucidation of host-pathogen protein-protein interactions to uncover mechanisms of host cell rewiring.Current opinion in microbiology · 2017Review
- Diversity of Mycobacterium tuberculosis across Evolutionary Scales.PLoS pathogens · 2015Article
- Characterization of effector and memory T cell subsets in the immune response to bovine tuberculosis in cattle.PloS one · 2015Article
- Genetic regulation of vesiculogenesis and immunomodulation in Mycobacterium tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Use of whole genome sequencing to determine the microevolution of Mycobacterium tuberculosis during an outbreak.PloS one · 2013Article
- Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages.Applied and environmental microbiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In spite of its highly immunogenic properties, Mycobacterium tuberculosis (Mtb) establishes persistent infection in otherwise healthy individuals, making it one of the most widespread and deadly human pathogens. Mtb's prolonged survival may reflect production of microbial factors that prevent even more vigorous immunity (quantitative effect) or that divert the immune response to a non-sterilizing mode (qualitative effect). Disruption of Mtb genes has produced a list of several dozen candidate immunomodulatory factors. Here we used robotic fluorescence microscopy to screen 10,100 loss-of-function transposon mutants of Mtb for their impact on the expression of promoter-reporter constructs for 12 host immune response genes in a mouse macrophage cell line. The screen identified 364 candidate immunoregulatory genes. To illustrate the utility of the candidate list, we confirmed the impact of 35 Mtb mutant strains on expression of endogenous immune response genes in primary macrophages. Detailed analysis focused on a strain of Mtb in which a transposon disrupts Rv0431, a gene encoding a conserved protein of unknown function. This mutant elicited much more macrophage TNFα, IL-12p40 and IL-6 in vitro than wild type Mtb, and was attenuated in the mouse. The mutant list provides a platform for exploring the immunobiology of tuberculosis, for example, by combining immunoregulatory mutations in a candidate vaccine strain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.