ArticleAutophagy2011
Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris.
Article in Autophagy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 41 citations in OpenAlex.
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- Completing Autophagy: Formation and Degradation of the Autophagic Body and Metabolite Salvage in Plants.International journal of molecular sciences · 2020Review
- Autophagic Induction Greatly EnhancesFrontiers in microbiology · 2018Article
- Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris.Applied microbiology and biotechnology · 2017Article
- Mechanistic insights into selective autophagy pathways: lessons from yeast.Nature reviews. Molecular cell biology · 2016Review
- Regulation of protein homeostasis in neurodegenerative diseases: the role of coding and non-coding genes.Cellular and molecular life sciences : CMLS · 2015Review
- Review
- Selective autophagy in budding yeast.Cell death and differentiation · 2013Review
- Role of macroautophagy in nutrient homeostasis during fungal development and pathogenesis.Cells · 2012Article
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- Autophagy: mechanism and physiological relevance 'brewed' from yeast studies.Frontiers in bioscience (Scholar edition) · 2012Review
- A mammalian autophagosome maturation mechanism mediated by TECPR1 and the Atg12-Atg5 conjugate.Molecular cell · 2012Article
- Pexophagy: the selective degradation of peroxisomes.International journal of cell biology · 2012Article
- Autophagy dysregulation in amyotrophic lateral sclerosis.Brain pathology (Zurich, Switzerland) · 2012Review
- A comprehensive glossary of autophagy-related molecules and processes (2nd edition).Autophagy · 2011Article
- The regulation of autophagy - unanswered questions.Journal of cell science · 2011Review
Corrections and comments
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Authors and funding
9 authors at 7 institutions in 5 countries.
Funding
Abstract
Autophagy-related (Atg) pathways deliver cytosol and organelles to the vacuole in double-membrane vesicles called autophagosomes, which are formed at the phagophore assembly site (PAS), where most of the core Atg proteins assemble. Atg28 is a component of the core autophagic machinery partially required for all Atg pathways in Pichia pastoris. This coiled-coil protein interacts with Atg17 and is essential for micropexophagy. However, the role of Atg28 in micropexophagy was unknown. We used the yeast two-hybrid system to search for Atg28 interaction partners from P. pastoris and identified a new Atg protein, named Atg35. The atg35∆ mutant was not affected in macropexophagy, cytoplasm-to-vacuole targeting or general autophagy. However, both Atg28 and Atg35 were required for micropexophagy and for the formation of the micropexophagic apparatus (MIPA). This requirement correlated with a stronger expression of both proteins on methanol and glucose. Atg28 mediated the interaction of Atg35 with Atg17. Trafficking of overexpressed Atg17 from the peripheral ER to the nuclear envelope was required to organize a peri-nuclear structure (PNS), the site of Atg35 colocalization during micropexophagy. In summary, Atg35 is a new Atg protein that relocates to the PNS and specifically regulates MIPA formation during micropexophagy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.