ArticleJournal of synchrotron radiation2011
Single-crystal Raman spectroscopy and X-ray crystallography at beamline X26-C of the NSLS.
Article in Journal of synchrotron radiation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Structural biology at the National Synchrotron Light Source II.Journal of synchrotron radiation · 2025Article
- A database overview of metal-coordination distances in metalloproteins.Acta crystallographica. Section D, Structural biology · 2024Article
- Characterizing metal-binding sites in proteins with X-ray crystallography.Nature protocols · 2018Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- Metalloprotein Crystallography: More than a Structure.Accounts of chemical research · 2016Article
- UV-Visible Absorption Spectroscopy Enhanced X-ray Crystallography at Synchrotron and X-ray Free Electron Laser Sources.Protein and peptide letters · 2016Review
- A nearly on-axis spectroscopic system for simultaneously measuring UV-visible absorption and X-ray diffraction in the SPring-8 structural genomics beamline.Journal of synchrotron radiation · 2016Article
- From protein structure to function via single crystal optical spectroscopy.Frontiers in molecular biosciences · 2015Review
- In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Macromolecular crystallography beamline X25 at the NSLS.Journal of synchrotron radiation · 2014Article
- A new on-axis micro-spectrophotometer for combining Raman, fluorescence and UV/Vis absorption spectroscopy with macromolecular crystallography at the Swiss Light Source.Journal of synchrotron radiation · 2013Article
- Quaternary ammonium oxidative demethylation: X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase.Journal of the American Chemical Society · 2012Article
- Correlated single-crystal electronic absorption spectroscopy and X-ray crystallography at NSLS beamline X26-C.Journal of synchrotron radiation · 2011Review
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Authors and funding
6 authors.
Funding
Abstract
Three-dimensional structures derived from X-ray diffraction of protein crystals provide a wealth of information. Features and interactions important for the function of macromolecules can be deduced and catalytic mechanisms postulated. Still, many questions can remain, for example regarding metal oxidation states and the interpretation of `mystery density', i.e. ambiguous or unknown features within the electron density maps, especially at ∼2 Å resolutions typical of most macromolecular structures. Beamline X26-C at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory (BNL), provides researchers with the opportunity to not only determine the atomic structure of their samples but also to explore the electronic and vibrational characteristics of the sample before, during and after X-ray diffraction data collection. When samples are maintained under cryo-conditions, an opportunity to promote and follow photochemical reactions in situ as a function of X-ray exposure is also provided. Plans are in place to further expand the capabilities at beamline X26-C and to develop beamlines at NSLS-II, currently under construction at BNL, which will provide users access to a wide array of complementary spectroscopic methods in addition to high-quality X-ray diffraction data.
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