ArticleJournal of bacteriology2011
Labeling and enzyme studies of the central carbon metabolism in Metallosphaera sedula.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- Article
- Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea.Environmental microbiology · 2025Article
- Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula.Extremophiles : life under extreme conditions · 2024Article
- Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways.Journal of advanced research · 2023Review
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Life in hot acid: a genome-based reassessment of the archaeal order Sulfolobales.Environmental microbiology · 2021Article
- In-Depth Computational Analysis of Natural and Artificial Carbon Fixation Pathways.Biodesign research · 2021Article
- Comparative Genomic Analysis Reveals the Metabolism and Evolution of the Thermophilic Archaeal GenusFrontiers in microbiology · 2020Article
- Increased chalcopyrite bioleaching capabilities of extremely thermoacidophilic Metallosphaera sedula inocula by mixotrophic propagation.Journal of industrial microbiology & biotechnology · 2019Article
- Evolutionary Steps in the Analytics of Primordial Metabolic Evolution.Life (Basel, Switzerland) · 2019Review
- Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.PloS one · 2019Article
- Isolation of nitrate-reducing bacteria from an offshore reservoir and the associated biosurfactant production.RSC advances · 2018Article
- Structural Insight into Substrate Specificity of 3-Hydroxypropionyl-Coenzyme A Dehydratase from Metallosphaera sedula.Scientific reports · 2018Article
- Integrated metabolism in sponge-microbe symbiosis revealed by genome-centered metatranscriptomics.The ISME journal · 2017Article
- Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system.The ISME journal · 2017Article
- Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Proteomics and comparative genomics of Nitrososphaera viennensis reveal the core genome and adaptations of archaeal ammonia oxidizers.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate COMetabolic engineering · 2016Article
- Integrated In Silico Analysis of Pathway Designs for Synthetic Photo-Electro-Autotrophy.PloS one · 2016Article
- Genome Sequencing ofFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallosphaera sedula (Sulfolobales, Crenarchaeota) uses the 3-hydroxypropionate/4-hydroxybutyrate cycle for autotrophic carbon fixation. In this pathway, acetyl-coenzyme A (CoA) and succinyl-CoA are the only intermediates that can be considered common to the central carbon metabolism. We addressed the question of which intermediate of the cycle most biosynthetic routes branch off. We labeled autotrophically growing cells by using 4-hydroxy[1-¹⁴C]butyrate and [1,4-¹³C₁]succinate, respectively, as precursors for biosynthesis. The labeling patterns of protein-derived amino acids verified the operation of the proposed carbon fixation cycle, in which 4-hydroxybutyrate is converted to two molecules of acetyl-CoA. The results also showed that major biosynthetic flux does not occur via acetyl-CoA, except for the formation of building blocks that are directly derived from acetyl-CoA. Notably, acetyl-CoA is not assimilated via reductive carboxylation to pyruvate. Rather, our data suggest that the majority of anabolic precursors are derived from succinyl-CoA, which is removed from the cycle via oxidation to malate and oxaloacetate. These C₄intermediates yield pyruvate and phosphoenolpyruvate (PEP). Enzyme activities that are required for forming intermediates from succinyl-CoA were detected, including enzymes catalyzing gluconeogenesis from PEP. This study completes the picture of the central carbon metabolism in autotrophic Sulfolobales by connecting the autotrophic carbon fixation cycle to the formation of central carbon precursor metabolites.
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