ArticlePlant & cell physiology2011
Light-regulated nuclear import and degradation of Arabidopsis phytochrome-A N-terminal fragments.
Article in Plant & cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Phytochrome A is required for light-inhibited germination of Aethionema arabicum seed.The New phytologist · 2025Article
- A base substitution in OsphyC disturbs its Interaction with OsphyB and affects flowering time and chlorophyll synthesis in rice.BMC plant biology · 2022Article
- Carotenoid Biosynthesis and Plastid Development in Plants: The Role of Light.International journal of molecular sciences · 2021Review
- Protein Kinase Activity of Phytochrome A Positively Correlates With Photoresponses in Arabidopsis.Frontiers in plant science · 2021Article
- Light Control of Salt-Induced Proline Accumulation Is Mediated by ELONGATED HYPOCOTYL 5 inFrontiers in plant science · 2019Article
- Article
- Evidence that phytochrome functions as a protein kinase in plant light signalling.Nature communications · 2016Article
- Lysine 206 in Arabidopsis phytochrome A is the major site for ubiquitin-dependent protein degradation.Journal of biochemistry · 2016Article
- The phytochrome B/phytochrome C heterodimer is necessary for phytochrome C-mediated responses in rice seedlings.PloS one · 2014Article
- Phosphatidic acid interacts with a MYB transcription factor and regulates its nuclear localization and function in Arabidopsis.The Plant cell · 2013Article
- The phytochrome gene family in soybean and a dominant negative effect of a soybean PHYA transgene on endogenous Arabidopsis PHYA.Plant cell reports · 2013Article
- Arabidopsis phytochrome a is modularly structured to integrate the multiple features that are required for a highly sensitized phytochrome.The Plant cell · 2012Article
- Photobodies in light signaling.Plant physiology · 2012Review
- Missense mutation in the amino terminus of phytochrome A disrupts the nuclear import of the photoreceptor.Plant physiology · 2012Article
- Phytochrome signaling mechanisms and the control of plant development.Trends in cell biology · 2011Review
- Phytochrome A-specific signaling in Arabidopsis thaliana.Plant signaling & behavior · 2011Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
The photoreceptor phytochrome-A (phyA) regulates germination and seedling establishment by mediating very low fluence (VLFR) and far-red high irradiance (FR-HIR) responses in Arabidopsis thaliana. In darkness, phyA homodimers exist in the biologically inactive Pr form and are localized in the cytoplasm. Light induces formation of the biologically active Pfr form and subsequent rapid nuclear import. PhyA Pfr, in contrast to the Pr form, is labile and has a half-life of ∼30 min. We produced transgenic plants in a phyA-201 null background that express the PHYA-yellow fluorescent protein (YFP) or the PHYA686-YFP-dimerization domain (DD) and PHYA686-YFP-DD-nuclear localization signal (NLS) or PHYA686-YFP-DD-nuclear exclusion signal (NES) fusion proteins. The PHYA686-YFP fusion proteins contained the N-terminal domain of phyA (686 amino acid residues), a short DD and the YFP. Here we report that (i) PHYA686-YFP-DD fusion protein is imported into the nucleus in a light-dependent fashion; (ii) neither of the PHYA686 fusion proteins is functional in FR-HIR and nuclear VLFR; and (iii) the phyA-dependent, blue light-induced inhibition of hypocotyl growth is mediated by the PHYA686-YFP-DD-NES but not by the PHYA686-YFP-DD-NLS and PHYA686-YFP-DD fusion proteins. We demonstrate that (i) light induces degradation of all PHYA N-terminal-containing fusion proteins and (ii) these N-terminal domain-containing fusion proteins including the constitutively nuclear PHYA686-YFP-DD-NLS and predominantly cytoplasmic PHYA686-YFP-DD-NES degrade at comparable rates but markedly more slowly than PHYA-YFP, whereas (iii) light-induced degradation of the native phyA is faster compared with PHYA-YFP.
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