ArticleBMC genomics2010
A molecular inversion probe assay for detecting alternative splicing.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection.Frontiers in plant science · 2017Review
- Molecular Inversion Probes for targeted resequencing in non-model organisms.Scientific reports · 2016Article
- Molecular inversion probe: a new tool for highly specific detection of plant pathogens.PloS one · 2014Article
- Quantification of type II procollagen splice forms using alternative transcript-qPCR (AT-qPCR).Matrix biology : journal of the International Society for Matrix BiologyArticle
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
backgroundA sensitive, high-throughput method for monitoring pre-mRNA splicing on a genomic scale is needed to understand the spectrum of alternatively spliced mRNA in human cells.
resultsWe adapted Molecular Inversion Probes (MIPs), a padlock-probe based technology, for the multiplexed capture and quantitation of individual splice events in human tissues. Individual MIP capture probes can be quantified using either DNA microarrays or high-throughput sequencing, which permits independent assessment of each spliced junction. Using our methodology we successfully identified 100% of our positive controls and showed that there is a strong correlation between the data from our alternative splicing MIP (asMIP) assay and quantitative PCR.
conclusionThe asMIP assay provides a sensitive, accurate and multiplexed means for measuring pre-mRNA splicing. Fully optimized, we estimate that the assay could accommodate a throughput of greater than 20,000 splice junctions in a single reaction. This would represent a significant improvement over existing technologies.
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