ArticleBiochemistry2011
A thermodynamic approach to the mechanism of cell-penetrating peptides in model membranes.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- A molecular dynamics study of cell-penetrating peptide transportan-10 (TP10): Binding, folding and insertion to transmembrane state in zwitterionic membrane.Biochimica et biophysica acta. Biomembranes · 2024Article
- Cell-Penetrating Peptides and Transportan.Pharmaceutics · 2021Review
- Charge Distribution Fine-Tunes the Translocation of α-Helical Amphipathic Peptides across Membranes.Biophysical journal · 2016Article
- Latarcins: versatile spider venom peptides.Cellular and molecular life sciences : CMLS · 2015Review
- Mechanism Matters: A Taxonomy of Cell Penetrating Peptides.Trends in biochemical sciences · 2015Review
- PE and PS Lipids Synergistically Enhance Membrane Poration by a Peptide with Anticancer Properties.Biophysical journal · 2015Article
- Peptides with the same composition, hydrophobicity, and hydrophobic moment bind to phospholipid bilayers with different affinities.The journal of physical chemistry. B · 2014Article
- Membrane-active peptides: binding, translocation, and flux in lipid vesicles.Biochimica et biophysica acta · 2014Review
- Effect of the aspartic acid D2 on the affinity of Polybia-MP1 to anionic lipid vesicles.European biophysics journal : EBJ · 2014Article
- Structure analysis and conformational transitions of the cell penetrating peptide transportan 10 in the membrane-bound state.PloS one · 2014Article
- Translocation of cationic amphipathic peptides across the membranes of pure phospholipid giant vesicles.Journal of the American Chemical Society · 2013Article
- Statistical analysis of peptide-induced graded and all-or-none fluxes in giant vesicles.Biophysical journal · 2013Article
- Hemolytic activity of membrane-active peptides correlates with the thermodynamics of binding to 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine bilayers.The Journal of membrane biology · 2013Article
- Hydrogen-bond energetics drive helix formation in membrane interfaces.Biochimica et biophysica acta · 2012Article
- Lipopolysaccharide neutralization by antimicrobial peptides: a gambit in the innate host defense strategy.Journal of innate immunity · 2012Review
- What determines the activity of antimicrobial and cytolytic peptides in model membranes.Biochemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report a first test of the hypothesis that the mechanism of antimicrobial, cytolytic, and amphipathic cell-penetrating peptides in model membranes is determined by the thermodynamics of insertion of the peptide into the lipid bilayer from the surface-associated state. Three peptides were designed with minimal mutations relative to the sequence of TP10W, the Y3W variant of transportan 10, which is a helical, amphipathic cell-penetrating peptide previously studied. Binding to 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) membranes and release of dye from those vesicles were assessed by stopped-flow fluorescence, and the secondary structure of the peptides on the membrane was determined by circular dichroism. The Gibbs energy of binding determined experimentally was in excellent agreement with that calculated using the Wimley-White interfacial hydrophobicity scale, taking into account the helical content of the membrane-associated peptide. Release of dye from POPC vesicles remained graded, as predicted by the hypothesis. More significantly, as the Gibbs energy of insertion into the bilayer became more unfavorable, which was estimated using the Wimley-White octanol hydrophobicity scale, dye release became slower, in quantitative agreement with the prediction.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.