ArticleJournal of the American Chemical Society2011
Protein thiocarboxylate-dependent methionine biosynthesis in Wolinella succinogenes.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Discovery of a novel methionine biosynthetic routeApplied and environmental microbiology · 2024Article
- Biochemical and Structural Characterization of OvoAACS catalysis · 2023Article
- Functional elucidation of TfuA in peptide backbone thioamidation.Nature chemical biology · 2021Article
- Filling gaps in bacterial amino acid biosynthesis pathways with high-throughput genetics.PLoS genetics · 2018Article
- Recent Advances in Our Understanding of the Biosynthesis of Sulfur Modifications in tRNAs.Frontiers in microbiology · 2018Article
- From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.Journal of the American Chemical Society · 2016Article
- Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.Molecules (Basel, Switzerland) · 2014Article
- Prokaryotic ubiquitin-like protein modification.Annual review of microbiology · 2014Review
- Different biosynthetic pathways to fosfomycin in Pseudomonas syringae and Streptomyces species.Antimicrobial agents and chemotherapy · 2012Article
- Thiamin biosynthesis: still yielding fascinating biological chemistry.Biochemical Society transactions · 2012Review
- A novel mechanism of sulfur transfer catalyzed by O-acetylhomoserine sulfhydrylase in the methionine-biosynthetic pathway of Wolinella succinogenes.Acta crystallographica. Section D, Biological crystallography · 2011Article
- Sulfur incorporation into biomolecules: recent advances.Critical reviews in biochemistry and molecular biologyReview
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2 authors at 1 institution in 1 country.
Funding
Abstract
Thiocarboxylated proteins are important intermediates in a variety of biochemical sulfide transfer reactions. Here we identify a protein thiocarboxylate-dependent methionine biosynthetic pathway in Wolinella succinogenes. In this pathway, the carboxy terminal alanine of a novel sulfur transfer protein, HcyS-Ala, is removed in a reaction catalyzed by a metalloprotease, HcyD. HcyF, an ATP-utilizing enzyme, catalyzes the adenylation of HcyS. HcyS acyl-adenylate then undergoes nucleophilic substitution by bisulfide produced by Sir to give the HcyS thiocarboxylate. This adds to O-acetylhomoserine to give HcyS-homocysteine in a PLP-dependent reaction catalyzed by MetY. HcyD-mediated hydrolysis liberates homocysteine. A final methylation completes the biosynthesis. The biosynthetic gene cluster also encodes the enzymes involved in the conversion of sulfate to sulfide suggesting that sulfate is the sulfur source for protein thiocarboxylate formation in this system.
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