ArticleArchives of virology2011
Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples.
Article in Archives of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
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- An Improved Workflow for the Quantification of Orthohantavirus Infection Using Automated Imaging and Flow Cytometry.Viruses · 2024Article
- Tropism ofViruses · 2023Article
- Characterization of a Panel of Cross-Reactive Hantavirus Nucleocapsid Protein-Specific Monoclonal Antibodies.Viruses · 2023Article
- Immunohistochemical diagnosis of human infectious diseases: a review.Diagnostic pathology · 2022Review
- Isolation and characterization of new Puumala orthohantavirus strains from Germany.Virus genes · 2020Article
- Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment.Pathogens (Basel, Switzerland) · 2020Article
- Article
- Human seroprevalence indicating hantavirus infections in tropical rainforests of Côte d'Ivoire and Democratic Republic of Congo.Frontiers in microbiology · 2015Article
- Antigenic properties of N protein of hantavirus.Viruses · 2014Review
- Article
- Generation of recombinant Schmallenberg virus nucleocapsid protein in yeast and development of virus-specific monoclonal antibodies.Journal of immunology research · 2014Article
- Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein.Virus genes · 2011Article
- Pathogenic old world hantaviruses infect renal glomerular and tubular cells and induce disassembling of cell-to-cell contacts.Journal of virology · 2011Article
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Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
Abstract
Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.
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