ArticleEuropean biophysics journal : EBJ2011
Fast uncoiling kinetics of F1C pili expressed by uropathogenic Escherichia coli are revealed on a single pilus level using force-measuring optical tweezers.
Article in European biophysics journal : EBJ, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
- Glycan-mediated adhesion mechanisms in antibiotic-resistant bacteria.BBA advances · 2025Article
- Three structural solutions for bacterial adhesion pilus stability and superelasticity.Structure (London, England : 1993) · 2023Article
- Unveiling molecular interactions that stabilize bacterial adhesion pili.Biophysical journal · 2022Article
- Impact of an alpha helix and a cysteine-cysteine disulfide bond on the resistance of bacterial adhesion pili to stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Role of Glycans in Bacterial Adhesion to Mucosal Surfaces: How Can Single-Molecule Techniques Advance Our Understanding?Microorganisms · 2018Review
- Theory for nonlinear dynamic force spectroscopy.European biophysics journal : EBJ · 2017Article
- The Nanomechanical Properties of Lactococcus lactis Pili Are Conditioned by the Polymerized Backbone Pilin.PloS one · 2016Article
- Structure, Function, and Assembly of Adhesive Organelles by Uropathogenic Bacteria.Microbiology spectrum · 2015Review
- Antibody-mediated disruption of the mechanics of CS20 fimbriae of enterotoxigenic Escherichia coli.Scientific reports · 2015Article
- Biomechanical and structural features of CS2 fimbriae of enterotoxigenic Escherichia coli.Biophysical journal · 2015Article
- Rigid multibody simulation of a helix-like structure: the dynamics of bacterial adhesion pili.European biophysics journal : EBJ · 2015Article
- Article
- Structure and function of enterotoxigenic Escherichia coli fimbriae from differing assembly pathways.Molecular microbiology · 2015Article
- Yielding elastic tethers stabilize robust cell adhesion.PLoS computational biology · 2014Article
- The shaft of the type 1 fimbriae regulates an external force to match the FimH catch bond.Biophysical journal · 2013Article
- Helix-like biopolymers can act as dampers of force for bacteria in flows.European biophysics journal : EBJ · 2012Article
- Impairment of the biomechanical compliance of P pili: a novel means of inhibiting uropathogenic bacterial infections?European biophysics journal : EBJ · 2012Article
- A structural basis for sustained bacterial adhesion: biomechanical properties of CFA/I pili.Journal of molecular biology · 2012Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uropathogenic Escherichia coli (UPEC) express various kinds of organelles, so-called pili or fimbriae, that mediate adhesion to host tissue in the urinary tract through specific receptor-adhesin interactions. The biomechanical properties of these pili have been considered important for the ability of bacteria to withstand shear forces from rinsing urine flows. Force-measuring optical tweezers have been used to characterize individual organelles of F1C type expressed by UPEC bacteria with respect to such properties. Qualitatively, the force-versus-elongation response was found to be similar to that of other types of helix-like pili expressed by UPEC, i.e., type 1, P, and S, with force-induced elongation in three regions, one of which represents the important uncoiling mechanism of the helix-like quaternary structure. Quantitatively, the steady-state uncoiling force was assessed as 26.4 ±1.4 pN, which is similar to those of other pili (which range from 21 pN for S(I) to 30 pN for type 1). The corner velocity for dynamic response (1,400 nm/s) was found to be larger than those of the other pili (400-700 nm/s for S and P pili, and 6 nm/s for type 1). The kinetics were found to be faster, with a thermal opening rate of 17 Hz, a few times higher than S and P pili, and three orders of magnitude higher than type 1. These data suggest that F1C pili are, like P and S pili, evolutionarily selected to primarily withstand the conditions expressed in the upper urinary tract.
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