ArticleMolecular biology reports2011
Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Genome-wide analysis of the bHLH gene family in Spatholobus suberectus identifies SsbHLH112 as a regulator of flavonoid biosynthesis.BMC plant biology · 2025Article
- Planarian RNAi knockdown: feeding once might just be enough.Frontiers in neuroscience · 2025Article
- Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Anoectochilus roxburghii.Molecular biology reports · 2012Article
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Authors and funding
6 authors.
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Abstract
It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian regeneration, three housekeeping genes, 18S rRNA, ACTB and DjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software. Expression levels of the Djsix-1 gene were studied in parallel with ACTB and DjEF2 both or each and 18S rRNA as reference during regeneration. The results showed that ACTB was the most stable expressed reference gene in the planarian regeneration.
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