ArticleMolecular biology reports2011
An efficient protocol for genetic transformation of watercress (Nasturtium officinale) using Agrobacterium rhizogenes.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 41 citations in OpenAlex.
- Construction of an Efficient Genetic Transformation System for Watercress (Plants (Basel, Switzerland) · 2023Article
- Variation in Glucosinolate Accumulation among Different Sprout and Seedling Stages of Broccoli (Plants (Basel, Switzerland) · 2022Article
- Phytochemical and Biological Activity Studies onMolecules (Basel, Switzerland) · 2020Article
- Reaction of indoles with aromatic fluoromethyl ketones: an efficient synthesis of trifluoromethyl(indolyl)phenylmethanols using KBeilstein journal of organic chemistry · 2020Article
- Biotechnological approaches in glucosinolate production.Journal of integrative plant biology · 2018Review
- Comparative analysis of glucosinolates and metabolite profiling of green and red mustard (3 Biotech · 2018Article
- Biosynthesis and bioactivity of glucosinolates and their production in plant in vitro cultures.Planta · 2017Review
- Synthesis of Ni(II) complexes bearing indole-based thiosemicarbazone ligands for interaction with biomolecules and some biological applications.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Article
- Article
- Induction of hairy roots byScientia horticulturae · 2016Article
- Effects of Watercress Containing Rutin and Rutin Alone on the Proliferation and Osteogenic Differentiation of Human Osteoblast-like MG-63 Cells.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2014Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Watercress (Nasturtium officinale) is a member of the Brassicaceae family and a rich source of glucosinolate, which has been shown to possess anticancer properties. To extract these compounds from N. officinale for study, a method was developed in which Agrobacterium rhizogenes was used to transfer DNA segments into plant genomes in order to produce hairy root cultures, which are a reliable source of plant compounds. The A. rhizogenes strain R1000 had the highest infection frequency and induces the most hairy roots per explant. Polymerase chain reaction and cytohistochemical staining methods were used to validate transgenic hairy roots from N. officinale. Glucosinolate from watercress hairy roots was separated and analyzed using high-performance liquid chromatography coupled to electrospray ionization mass spectrometry. Indolic glucosinolates, including glucobrassicin (0.01-0.02 μmol/g of DW) and 4-methoxyglucobrassicin (0.06-0.18 μmol/g of DW), as well as aromatic glucosinolate (gluconasturtiin) (0.06-0.21 μmol/g of DW), were identified virtually identical or more in transformed than wild type roots of N. officinale. Hairy root culture of watercress is a valuable approach for future efforts in the metabolic engineering of glucosinolate biofortification in plants, particularly, because indolic glucosinolates are the precursors of a potent cancer chemopreventive agent (indole-3-carbinol).
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