ArticleMolecular biology reports2011
Validation and application of reference genes for quantitative gene expression analyses in various tissues of Bupleurum chinense.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 27 citations in OpenAlex.
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- Suitable concentrations of brassinolide application enhances growth performance and saikosaponin biosynthesis by transcriptional activation of triterpenoid pathway genes inFrontiers in plant science · 2025Article
- Tumor-Educated Platelet miR-18a-3p as a Novel Liquid-Biopsy Biomarker for Early Diagnosis and Chemotherapy Efficacy Monitoring in Nasopharyngeal Carcinoma.Frontiers in oncology · 2021Article
- Selection and validation of reference genes for quantitative gene expression analyses in various tissues and seeds at different developmental stages inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Selection of Reference Genes for Real-Time Quantitative PCR in Pinus massoniana Post Nematode Inoculation.PloS one · 2016Article
- Identification of endogenous controls for analyzing serum exosomal miRNA in patients with hepatitis B or hepatocellular carcinoma.Disease markers · 2015Article
- Immunocytochemical localization of saikosaponin-d in vegetative organs of Bupleurum scorzonerifolium Willd.Botanical studies · 2013Article
- Article
- Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa.Molecular breeding : new strategies in plant improvement · 2012Article
- Characterization of reference genes for quantitative real-time PCR analysis in various tissues of Anoectochilus roxburghii.Molecular biology reports · 2012Article
- Distribution and expression characteristics of triterpenoids and OSC genes in white birch (Betula platyphylla suk.).Molecular biology reports · 2012Article
- Selection of reference genes for quantitative real-time RT-PCR analysis in citrus.Molecular biology reports · 2012Article
- Validation of reference genes for quantitative expression analysis by real-time rt-PCR in four lepidopteran insects.Journal of insect science (Online) · 2012Article
- Transcriptome analysis of Bupleurum chinense focusing on genes involved in the biosynthesis of saikosaponins.BMC genomics · 2011Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
It is crucial to select stable references in gene expression analyses using quantitative real-time PCR (qRT-PCR). In this work, seven frequently used reference genes, 18S, Actin, EF1α, α-tubulin, β-tubulin, Cyclophilin and Cytoplasmic ribosomal protein L2 (L2), from Bupleurum chinense DC. were evaluated as the internal control in five tissues, roots, stems, leaves, flowers and fruits, before tissue specific gene expression assays. The results showed that β-tubulin was the most stable and reliable reference gene among the seven candidate genes in the measured tissues. The expression levels of four genes involved in saikosaponins (the pharmacological active compounds of B. chinense) biosynthesis, HMGR, IPPI, FPS and β-AS, were assayed with β-tubulin as the internal control in the five tissues. All the four genes were expressed in the five tissues with different profiles and HMGR in the order of roots > flowers, stems and leaves > fruits, IPPI of stems > leaves and fruits > roots and flowers, FPS of flowers > fruits > stems and roots > leaves and β-AS of roots > flowers, stems and fruits > leaves. The genes of FPS and β-AS were expressed predominantly in flowers and roots, respectively. This study may provide a suitable internal control for quantitative gene expression assays in various tissues and give insight into the tissue expression profiles of four saikosaponins biosynthesis-involved genes of medicinal B. chinense.
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