ArticlePlant cell reports2011
Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Defining Phytochemical Metabolomes of Somatic HybridsMolecules (Basel, Switzerland) · 2025Article
- Article
- Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions.Biology · 2022Review
- Effect of Cryopreservation Method Supported with Biochemical Analyses in the Axillary Bud of Jewel Orchid,Plants (Basel, Switzerland) · 2022Article
- Article
- Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants.Plants (Basel, Switzerland) · 2021Review
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Androgenic-Induced Transposable Elements Dependent Sequence Variation in Barley.International journal of molecular sciences · 2021Article
- Comparative Study of the Genetic and Biochemical Variability ofInternational journal of molecular sciences · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- Effect of Cryopreservation on the Ex Vitro Establishment of Olive Plants Regenerated via Somatic Embryogenesis.Plants (Basel, Switzerland) · 2021Article
- Effect of Cryopreservation on Olive (Plants (Basel, Switzerland) · 2020Article
- Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation.International journal of molecular sciences · 2020Review
- Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley.Plant molecular biology · 2020Article
- Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants.Frontiers in plant science · 2020Article
- Somatic Embryogenesis in the Family Gentianaceae and Its Biotechnological Application.Frontiers in plant science · 2019Review
- Somatic embryogenesis in ferns: a new experimental system.Plant cell reports · 2015Article
- Extended metAFLP approach in studies of tissue culture induced variation (TCIV) in triticale.Molecular breeding : new strategies in plant improvement · 2014Article
- Cryopreservation of in vitro-produced Rhizophagus species has minor effects on their morphology, physiology, and genetic stability.Mycorrhiza · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The embryogenic cell suspension culture of Gentiana cruciata, cryopreserved by the encapsulation/dehydration method, survived both short- (48 h) and long-term (1.5 years) cryostorage with more than 80% viability. To assess the influence of cryotreatments on the embryogenic potential, a proembryogenic mass was encapsulated and exposed to the following treatments: (1) osmotic dehydration (OD), (2) OD + air desiccation (AD) and (3) OD + AD + cryostorage (LN). The somatic embryogenesis efficiency increased ten times after osmotic dehydration. The AD and LN cryotreatments did not cause any significant alterations in somatic embryo production. We monitored the (epi)genetic stability of 288 regenerants derived from: non-cryotreated, short-term, and long-term cryostored tissue using metAFLP markers and ten primer combinations. Changes in the sequence and DNA methylation levels were studied by subjecting the DNA to digestion with two pairs of isoschisomer restriction enzymes (KpnI/MseI and Acc65I/MseI). Two new AFLP unique DNA fragments at the DNA sequence level, with no differences at the methylation level, were found between regenerants derived from cryopreserved tissue, compared with the non-cryotreated controls. The Acc65I/MseI methylation levels for the three groups of regenerants were not significantly different. Cluster analysis was capable of identifying a number of sub-clusters. Only one of the sub-clusters comprises almost all regenerants derived from non-cryotreated and short-term cryostored tissue. Plantlets derived from long-term cryostored tissue were grouped into separate clusters. The observed AFLP alterations did not appear to be associated with the use of cryopreservation, but were probably related to the process of in vitro culture.
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