ArticleWorld journal of hepatology2010
Innovative immunohistochemistry identifies MMP-9 expressing macrophages at the invasive front of murine HCC.
Article in World journal of hepatology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Immunotherapy for Hepatocellular Carcinoma in the Setting of Liver Transplantation: A Review.International journal of molecular sciences · 2023Review
- Review
- Myeloid-derived suppressor cells in gastrointestinal cancers: A systemic review.World journal of gastrointestinal oncology · 2021Review
- Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Noninvasive Assessment of Liver Fibrosis: Current and Future Clinical and Molecular Perspectives.International journal of molecular sciences · 2020Review
- β-Sitosterol attenuates carbon tetrachloride-induced oxidative stress and chronic liver injury in rats.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Role of nonresolving inflammation in hepatocellular carcinoma development and progression.NPJ precision oncology · 2018Review
- Hepatic B cell leukemia-3 suppresses chemically-induced hepatocarcinogenesis in mice through altered MAPK and NF-κB activation.Oncotarget · 2017Article
- Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice.Oncotarget · 2017Article
- Article
- Protective Effect of Boiled and Freeze-dried Mature Silkworm Larval Powder Against Diethylnitrosamine-induced Hepatotoxicity in Mice.Journal of cancer prevention · 2016Article
- TLR4 Deficiency Protects against Hepatic Fibrosis and Diethylnitrosamine-Induced Pre-Carcinogenic Liver Injury in Fibrotic Liver.PloS one · 2016Article
- Hepatic macrophages in liver fibrosis: pathogenesis and potential therapeutic targets.BMJ open gastroenterology · 2016Article
- Myeloid cells in hepatocellular carcinoma.Hepatology (Baltimore, Md.) · 2015Review
- Microenvironmental regulation of therapeutic response in cancer.Trends in cell biology · 2015Review
- Targeting the tumor stroma in hepatocellular carcinoma.World journal of hepatology · 2015Review
- Article
- A multicenter study on expressions of vascular endothelial growth factor, matrix metallopeptidase-9 and tissue inhibitor of metalloproteinase-2 in oral and maxillofacial squamous cell carcinoma.Iranian Red Crescent medical journal · 2014Article
- Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo.Neuroscience bulletin · 2013Article
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6 authors.
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Abstract
aimTo investigate the proteolytic contribution of tumor-associated macrophages (TAM) in tumor invasion, we analyzed whether TAM at the invasive front of small HCC in Abcb4(-/-)-mice show an enhanced expression of MMP-9.
methodsLiver cryosections of the hepatocellular carcinoma (HCC) invasive front from 12 mo old Abcb4(-/-)-mice were stained for collagen type I and MMP-9 using Alexa488 and Alexa568 labeled secondary antibodies. Afterwards, the Alexa568 dye was bleached and the macrophage marker F4/80 was visualized using Alexa568 labeled secondary antibodies. Finally, photographs of the invasive tumor front were digitally overlaid and analyzed.
resultsAfter complete bleaching of the primary dye, specific fluorescence staining of a third antigen, here F4/80, was successfully performed on the same histological section. With this method, we were able to identify conglomerates of matrix metalloproteinase (MMP-9) expressing macrophages within the tumor capsule of HCC.
conclusionMMP-9 expressing macrophages are involved in matrix remodelling at the invasive tumor front of HCC. The described staining protocol provides a simple yet powerful extension of conventional immuno-histochemistry, facilitating visualization of at least three different antigens plus nuclei in one single histological section.
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