ReviewMolecular biology of the cell2010
Toward quantitative "in vivo biochemistry" with fluorescence fluctuation spectroscopy.
Review in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization.Bio-protocol · 2020Article
- Quantitative modeling of self-oligomerization of proteins in the nuclear envelope by fluorescence fluctuation analysis.Analytical biochemistry · 2019Article
- Protein oligomerization and mobility within the nuclear envelope evaluated by the time-shifted mean-segmented Q factor.Methods (San Diego, Calif.) · 2019Article
- Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.Communications biology · 2019Article
- A flow-proteometric platform for analyzing protein concentration (FAP): Proof of concept for quantification of PD-L1 protein in cells and tissues.Biosensors & bioelectronics · 2018Article
- Fluorescence fluctuation spectroscopy reveals differential SUN protein oligomerization in living cells.Molecular biology of the cell · 2018Article
- Oligomeric state regulated trafficking of human platelet-activating factor acetylhydrolase type-II.Biochimica et biophysica acta · 2015Article
- Organelle-based aggregation and retention of damaged proteins in asymmetrically dividing cells.Cell · 2014Article
- Measuring protein dynamics in live cells: protocols and practical considerations for fluorescence fluctuation microscopy.Journal of biomedical optics · 2014Article
- The SUN protein Mps3 controls Ndc1 distribution and function on the nuclear membrane.The Journal of cell biology · 2014Article
- Imaging methodologies for systems biology.Cell adhesion & migration · 2014Review
- Determining absolute protein numbers by quantitative fluorescence microscopy.Methods in cell biology · 2014Article
- Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization.Molecular biology of the cell · 2013Article
- Non-uniform membrane diffusion enables steady-state cell polarization via vesicular trafficking.Nature communications · 2013Article
- Time-integrated fluorescence cumulant analysis and its application in living cells.Methods in enzymology · 2013Article
- An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging.Journal of the American Chemical Society · 2012Article
- Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.Nature cell biology · 2012Article
- Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Quantitative description of protein dynamics and interactions in vivo with temporal and spatial resolution is a key step in dissecting molecular mechanisms in cell biology. Fluorescence fluctuation spectroscopy (FFS) has recently emerged as a powerful in vivo tool for assessing molecular concentration and movement and formation of hetero- and homo-oligomeric complexes. This article discusses point FFS-based analysis methods that have proven useful to cell biologists, focusing on the kinds of information they provide, their pros and cons, and the basic instrumentation required. Along the way, we describe briefly a few recent examples where these analyses have helped address important biological questions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.