ArticleRNA (New York, N.Y.)2011
Competing RNA secondary structures are required for mutually exclusive splicing of the Dscam exon 6 cluster.
Article in RNA (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Regulatory paradigm of Dscam1 stochastic alternative splicing through conserved long-range RNA structures.Nucleic acids research · 2026Article
- Ancestral intronic splicing regulatory elements in the SCNbioRxiv : the preprint server for biology · 2026Article
- RNA structure in alternative splicing regulation: from mechanism to therapy.Acta biochimica et biophysica Sinica · 2024Review
- Causes, functions, and therapeutic possibilities of RNA secondary structure ensembles and alternative states.Cell chemical biology · 2024Review
- Hidden RNA pairings counteract the "first-come, first-served" splicing principle to drive stochastic choice inScience advances · 2022Article
- Reverse complementary matches simultaneously promote both back-splicing and exon-skipping.BMC genomics · 2021Article
- Complex RNA Secondary Structures Mediate Mutually Exclusive Splicing of ColeopteraFrontiers in genetics · 2021Article
- May the Odds Be Ever in Your Favor: Non-deterministic Mechanisms Diversifying Cell Surface Molecule Expression.Frontiers in cell and developmental biology · 2021Review
- RNA secondary structures inRNA (New York, N.Y.) · 2020Article
- Alternate RNA Structures.Cold Spring Harbor perspectives in biology · 2020Review
- Srrm234, but not canonical SR and hnRNP proteins, drive inclusion ofRNA (New York, N.Y.) · 2019Article
- Revisiting Dscam diversity: lessons from clustered protocadherins.Cellular and molecular life sciences : CMLS · 2019Review
- Competing RNA pairings in complex alternative splicing of a 3' variable region.RNA (New York, N.Y.) · 2018Article
- Article
- Review
- The determinants of alternative RNA splicing in human cells.Molecular genetics and genomics : MGG · 2017Review
- Role and convergent evolution of competing RNA secondary structures in mutually exclusive splicing.RNA biology · 2017Article
- RNA structure in splicing: An evolutionary perspective.RNA biology · 2016Review
- MECHANISMS IN ENDOCRINOLOGY: Alternative splicing: the new frontier in diabetes research.European journal of endocrinology · 2016Review
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Alternative splicing of eukaryotic pre-mRNAs is an important mechanism for generating proteome diversity and regulating gene expression. The Drosophila melanogaster Down Syndrome Cell Adhesion Molecule (Dscam) gene is an extreme example of mutually exclusive splicing. Dscam contains 95 alternatively spliced exons that potentially encode 38,016 distinct mRNA and protein isoforms. We previously identified two sets of conserved sequence elements, the docking site and selector sequences in the Dscam exon 6 cluster, which contains 48 mutually exclusive exons. These elements were proposed to engage in competing RNA secondary structures required for mutually exclusive splicing, though this model has not yet been experimentally tested. Here we describe a new system that allowed us to demonstrate that the docking site and selector sequences are indeed required for exon 6 mutually exclusive splicing and that the strength of these RNA structures determines the frequency of exon 6 inclusion. We also show that the function of the docking site has been conserved for ~500 million years of evolution. This work demonstrates that conserved intronic sequences play a functional role in mutually exclusive splicing of the Dscam exon 6 cluster.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.