Evidence map›Paper›PMID 21158483›Full record

ArticleACS nano2011

Enhancement of single-molecule fluorescence signals by colloidal silver nanoparticles in studies of protein translation.

Shashank Bharill, Chunlai Chen, Benjamin Stevens, Jaskiran Kaur, Zeev Smilansky, Wlodek Mandecki, Ignacy Gryczynski, Zygmunt Gryczynski, Barry S Cooperman, Yale E Goldman

Open access · greenAbstract read
In one paragraph

Article in ACS nano, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 47 citations in OpenAlex.

  1. Metal-Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Plasmonic Nanoparticles: Advanced Researches (II).Advances in experimental medicine and biology · 2021
    Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Stability of silver nanoparticle monolayers determined by in situ streaming potential measurements.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2013
    Article
  16. Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Shashank BharillDepartment of Molecular Biology and Immunology, UNTHSC, Fort Worth, Texas 76107, United States.
Chunlai Chen
Benjamin Stevens
Jaskiran Kaur
Zeev Smilansky
Wlodek Mandecki
Ignacy Gryczynski
Zygmunt Gryczynski
Barry S Cooperman
Yale E Goldman
University of Pennsylvania · USUniversity of North Texas · USUniversity of North Texas Health Science Center · US

Funding

Single Molecule Dynamics of mRNA TranslationR01GM080376 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI COOPERMAN, BARRY S., GOLDMAN, YALE E · 2008 to 2016
$2.5M
Ribosome-based Single Molecule Method to Acquire Sequence Data from GenomesR01HG004364 · NHGRI · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI MANDECKI, WLODEK · 2007 to 2010
$1.9M
NHGRI NIH HHS HG004364NHGRI NIH HHS R01 HG004364NIGMS NIH HHS GM080376NIGMS NIH HHS R01 GM080376
6 · The paper itself

Abstract

Metal-enhanced fluorescence (MEF) increased total photon emission of Cy3- and Cy5-labeled ribosomal initiation complexes near 50 nm silver particles 4- and 5.5-fold, respectively. Fluorescence intensity fluctuations above shot noise, at 0.1-5 Hz, were greater on silver particles. Overall signal-to-noise ratio was similar or slightly improved near the particles. Proximity to silver particles did not compromise ribosome function, as measured by codon-dependent binding of fluorescent tRNA, dynamics of fluorescence resonance energy transfer between adjacent tRNAs in the ribosome, and tRNA translocation induced by elongation factor G.

Indexed as

Fluorescence Resonance Energy TransferProtein BiosynthesisBase SequenceCarbocyaninesColloidsMetal NanoparticlesPhotobleachingPhotonsRibosomesRNA, MessengerRNA, TransferSilverCarbocyaninesColloidscyanine dye 3cyanine dye 5RNA, MessengerRNA, TransferSilver

Identifiers

PMID21158483
PMCPMC3049198
OpenAlexW2048912747

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.