ArticleWorld journal of gastroenterology2010
Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients.
Article in World journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Circulating Tumour Cells as Prognostic Biomarkers in Colorectal Cancer: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Evaluating the efficacy of PARP inhibitor in ARID1A-deficient colorectal cancer: ACancer biomarkers : section A of Disease markers · 2025Article
- ARHGAP25 expression in colorectal cancer as a biomarker associated with favorable prognosis.Molecular and clinical oncology · 2022Article
- Coupling of serum CK20 and hyper-methylatedAging · 2021Article
- Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers.Micromachines · 2020Review
- TOMM20 as a potential therapeutic target of colorectal cancer.BMB reports · 2019Article
- Clinical significance and diagnostic capacity of serum TK1, CEA, CA 19-9 and CA 72-4 levels in gastric and colorectal cancer patients.Journal of Cancer · 2018Article
- Colorectal Cancer Blood-Based Biomarkers.Gastroenterology research and practice · 2017Review
- Death receptor 5 (DR5) and a 5-gene apoptotic biomarker panel with significant differential diagnostic potential in colorectal cancer.Scientific reports · 2016Article
- Role of specific DNA mutations in the peripheral blood of colorectal cancer patients for the assessment of tumor stage and residual disease following tumor resection.Oncology letters · 2016Article
- Use of blood-based biomarkers for early diagnosis and surveillance of colorectal cancer.World journal of gastrointestinal oncology · 2014Review
- Combined detection tumor markers for diagnosis and prognosis of gallbladder cancer.World journal of gastroenterology · 2014Review
- Evaluation of the adenocarcinoma-associated gene AGR2 and the intestinal stem cell marker LGR5 as biomarkers in colorectal cancer.International journal of molecular sciences · 2012Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a multiplex reverse transcription polymerase chain reaction (RT-PCR) method detecting circulating tumor cells in the peripheral blood of colorectal cancer (CRC) patients.
methodsPeripheral blood samples were collected from 88 CRC patients and 40 healthy individuals from the blood donors' clinic and subsequently analyzed by multiplex RT-RCR for the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and epidermal growth factor receptor (EGFR) mRNA. The analysis involved determining the detection rates of CEA, CK20 and EGFR transcripts vs disease stage and overall survival. Median follow-up period was 19 mo (range 8-28 mo).
resultsRates of CEA, CK20 and EGFR detection in CRC patients were 95.5%, 78.4% and 19.3%, respectively. CEA transcripts were detected in 3 healthy volunteer samples (7.5%), whereas all control samples were tested negative for CK20 and EGFR transcripts. The increasing number of positive detections for CEA, CK20 and EGFR transcripts in each blood sample was positively correlated with Astler-Coller disease stage (P < 0.001) and preoperative serum levels of CEA (P = 0.029) in CRC patients. Data analysis using Kaplan-Meier estimator documented significant differences in the overall survival of the different CRC patient groups as formed according to the increasing number of positivity for CEA, CK20 and EGFR transcripts.
conclusionThese data suggest that multiplex RT-PCR assay can provide useful information concerning disease stage and overall survival of CRC patients.
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