ArticlemBio2010
The Hyb hydrogenase permits hydrogen-dependent respiratory growth of Salmonella enterica serovar Typhimurium.
Article in mBio, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people.Nature microbiology · 2025Article
- Selective inhibition of NikA mediated Ni(II) import in E. coli by the Indium(III)-EDTA complex.Metallomics : integrated biometal science · 2025Article
- A two-hybrid system reveals previously uncharacterized protein-protein interactions within the Helicobacter pylori NIF iron-sulfur maturation system.Scientific reports · 2021Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Enhanced Growth of Pilin-Deficient Geobacter sulfurreducens Mutants in Carbon Poor and Electron Donor Limiting Conditions.Microbial ecology · 2019Article
- Nickel chelation therapy as an approach to combat multi-drug resistant enteric pathogens.Scientific reports · 2019Article
- The structure of hydrogenase-2 fromThe Biochemical journal · 2018Article
- Article
- First insights into the pleiotropic role of vrf (yedF), a newly characterized gene of Salmonella Typhimurium.Scientific reports · 2017Article
- Human Gut Microbiota: Toward an Ecology of Disease.Frontiers in microbiology · 2017Review
- Anaerobic Formate and Hydrogen Metabolism.EcoSal Plus · 2016Review
- Exploiting host immunity: the Salmonella paradigm.Trends in immunology · 2015Review
- Host hydrogen rather than that produced by the pathogen is important for Salmonella enterica serovar Typhimurium virulence.Infection and immunity · 2015Article
- Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization.Microbial biotechnology · 2014Article
- Commensal bacteria mediated defenses against pathogens.Current opinion in immunology · 2014Review
- How the structure of the large subunit controls function in an oxygen-tolerant [NiFe]-hydrogenase.The Biochemical journal · 2014Article
- RNA sequencing reveals differences between the global transcriptomes of Salmonella enterica serovar enteritidis strains with high and low pathogenicities.Applied and environmental microbiology · 2014Article
- Salmonella Typhimurium strain ATCC14028 requires H2-hydrogenases for growth in the gut, but not at systemic sites.PloS one · 2014Article
- A link between gut community metabolism and pathogenesis: molecular hydrogen-stimulated glucarate catabolism aids Salmonella virulence.Open biology · 2013Article
- Roles of H2 uptake hydrogenases in Shigella flexneri acid tolerance.Microbiology (Reading, England) · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Salmonella enterica serovar Typhimurium contains three distinct respiratory hydrogenases, all of which contribute to virulence. Addition of H(2) significantly enhanced the growth rate and yield of S. Typhimurium in an amino acid-containing medium; this occurred with three different terminal respiratory electron acceptors. Based on studies with site-specific double-hydrogenase mutant strains, most of this H(2)-dependent growth increase was attributed to the Hyb hydrogenase, rather than to the Hya or Hyd respiratory H(2)-oxidizing enzymes. The wild type strain with H(2) had 4.0-fold greater uptake of (14)C-labeled amino acids over a period of minutes than did cells incubated without H(2). The double-uptake hydrogenase mutant containing only the Hyb hydrogenase transported amino acids H(2) dependently like the wild type. The Hyb-only-containing strain produced a membrane potential comparable to that of the wild type. The H(2)-stimulated amino acid uptake of the wild type and the Hyb-only strain was inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazone but was less affected by the ATP synthase inhibitor sodium orthovanadate. In the wild type, proteins TonB and ExbD, which are known to couple proton motive force (PMF) to transport processes, were induced by H(2) exposure, as were the genes corresponding to these periplasmic PMF-coupling factors. However, studies on tonB and exbD single mutant strains could not confirm a major role for these proteins in amino acid transport. The results link H(2) oxidation via the Hyb enzyme to growth, amino acid transport, and expression of periplasmic proteins that facilitate PMF-mediated transport across the outer membrane.
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