ArticlemBio2010
RegB kinase activity is controlled in part by monitoring the ratio of oxidized to reduced ubiquinones in the ubiquinone pool.
Article in mBio, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 63 citations in OpenAlex.
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- A cryptic transcription factor regulates Caulobacter adhesin development.PLoS genetics · 2022Article
- PioABC-Dependent Fe(II) Oxidation during Photoheterotrophic Growth on an Oxidized Carbon Substrate Increases Growth Yield.Applied and environmental microbiology · 2022Article
- The Response Regulator RegA Is a Copper Binding Protein That Covalently Dimerizes When Exposed to Oxygen.Microorganisms · 2022Article
- RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence.PLoS pathogens · 2021Article
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- Engineering Photosynthetic Bioprocesses for Sustainable Chemical Production: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Induction of theFrontiers in microbiology · 2020Article
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- Regulation of bacterial surface attachment by a network of sensory transduction proteins.PLoS genetics · 2019Article
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- Characterization of a Glycyl Radical Enzyme Bacterial Microcompartment Pathway inJournal of bacteriology · 2019Article
- Dual RNA-seq of Xanthomonas oryzae pv. oryzicola infecting rice reveals novel insights into bacterial-plant interaction.PloS one · 2019Article
- Differing isoforms of the cobalamin binding photoreceptor AerR oppositely regulate photosystem expression.eLife · 2018Article
- Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production inFrontiers in microbiology · 2018Article
- Transcriptomic analysis of aerobic respiratory and anaerobic photosynthetic states inMicrobial genomics · 2017Article
- Atypical modes of bacterial histidine kinase signaling.Molecular microbiology · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RegB is a membrane-spanning sensor kinase responsible for redox regulation of a wide variety of metabolic processes in numerous proteobacterial species. Here we show that full-length RegB purified from Escherichia coli membranes contains bound ubiquinone. Four conserved residues in the membrane-spanning domain of RegB are shown to have important roles in ubiquinone binding in vitro and redox sensing in vivo. Isothermal titration calorimetry measurements, coupled with kinase assays under oxidizing and reducing conditions, indicate that RegB weakly binds both oxidized ubiquinone and reduced ubiquinone (ubiquinol) with nearly equal affinity and that oxidized ubiquinone inhibits kinase activity without promoting a redox reaction. We propose a model in which ubiquinone/ubiquinol bound to RegB readily equilibrates with ubiquinones/ubiquinols in the membrane, allowing the kinase activity to be tuned by the redox state of the ubiquinone pool. This noncatalytic role of ubiquinone in controlling RegB activity is distinct from that of other known ubiquinone-binding proteins, which use ubiquinone as an electron donor or acceptor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.