ArticleBiophysical journal2010
Nanotribology results show that DNA forms a mechanically resistant 2D network in metaphase chromatin plates.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Hypothesis: The opposing pulling forces exerted by spindle microtubules can cause sliding of chromatin layers and facilitate sister chromatid resolution.Frontiers in genetics · 2023Article
- Frozen-hydrated chromatin from metaphase chromosomes has an interdigitated multilayer structure.The EMBO journal · 2019Article
- ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells.Science (New York, N.Y.) · 2017Article
- Stacked thin layers of metaphase chromatin explain the geometry of chromosome rearrangements and banding.Scientific reports · 2015Article
- The energy components of stacked chromatin layers explain the morphology, dimensions and mechanical properties of metaphase chromosomes.Journal of the Royal Society, Interface · 2014Article
- Self-assembly of thin plates from micrococcal nuclease-digested chromatin of metaphase chromosomes.Biophysical journal · 2012Article
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Authors and funding
5 authors.
Funding
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Abstract
In a previous study, we found that metaphase chromosomes are formed by thin plates, and here we have applied atomic force microscopy (AFM) and friction force measurements at the nanoscale (nanotribology) to analyze the properties of these planar structures in aqueous media at room temperature. Our results show that high concentrations of NaCl and EDTA and extensive digestion with protease and nuclease enzymes cause plate denaturation. Nanotribology studies show that native plates under structuring conditions (5 mM Mg2+) have a relatively high friction coefficient (μ≈0.3), which is markedly reduced when high concentrations of NaCl or EDTA are added (μ≈0.1). This lubricant effect can be interpreted considering the electrostatic repulsion between DNA phosphate groups and the AFM tip. Protease digestion increases the friction coefficient (μ≈0.5), but the highest friction is observed when DNA is cleaved by micrococcal nuclease (μ≈0.9), indicating that DNA is the main structural element of plates. Whereas nuclease-digested plates are irreversibly damaged after the friction measurement, native plates can absorb kinetic energy from the AFM tip without suffering any damage. These results suggest that plates are formed by a flexible and mechanically resistant two-dimensional network which allows the safe storage of DNA during mitosis.
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