ArticleCurrent protocols in nucleic acid chemistry2010
Determination of nucleic acid hydration using osmotic stress.
Article in Current protocols in nucleic acid chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Conformational Morphing by a DNA Analogue Featuring 7-Deazapurines and 5-Halogenpyrimidines and the Origins of Adenine-Tract Geometry.Biochemistry · 2023Article
- Roles of the amino group of purine bases in the thermodynamic stability of DNA base pairing.Molecules (Basel, Switzerland) · 2014Article
- Synthesis, biophysical studies and RNA interference activity of RNA having three consecutive amide linkages.Organic & biomolecular chemistry · 2014Article
- Polyethylene glycol binding alters human telomere G-quadruplex structure by conformational selection.Nucleic acids research · 2013Article
- 2'-Fluoro RNA shows increased Watson-Crick H-bonding strength and stacking relative to RNA: evidence from NMR and thermodynamic data.Angewandte Chemie (International ed. in English) · 2012Article
- Hydration changes upon DNA folding studied by osmotic stress experiments.Biophysical journal · 2012Article
- Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.Nucleic acids research · 2011Article
- Amides as excellent mimics of phosphate linkages in RNA.Angewandte Chemie (International ed. in English) · 2011Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Water plays an important role in structure and molecular recognition of biopolymers. Understanding hydration of biopolymers is a significant problem in structural chemistry and biology. However, hydration is a dynamic process that is difficult to study. While X-ray crystallography, NMR, and molecular modeling have provided structural detail on nucleic acid hydration and valuable insights into water dynamics, the thermodynamic contribution of water molecules to conformational equilibria and recognition of nucleic acids remains poorly understood. This unit describes a thermodynamic analysis of nucleic acid hydration using osmotic stress. Osmotic stress monitors the depression of melting temperature upon decreasing water activity, and calculates the number of thermodynamically unique water molecules associated with the double helix and released from single strands upon melting. Comparison of the number of water molecules released upon melting of nucleic acids with different sequences and chemical modifications provides insights that complement and enhance information obtained by other methods.
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