ArticleInternational journal of biological sciences2010
Non-homologous end joining plays a key role in transgene concatemer formation in transgenic zebrafish embryos.
Article in International journal of biological sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Quantitative Evaluation of NR Locus-Targeted Nuclear Transformation inJournal of microbiology and biotechnology · 2026Article
- Review
- Progress in Gene-Editing Technology of Zebrafish.Biomolecules · 2021Review
- DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote.Nucleic acids research · 2020Article
- One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish.eLife · 2019Article
- CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes.Molecules and cells · 2018Review
- Injection of an SV40 transcriptional terminator causes embryonic lethality: a possible zebrafish model for screening nonhomologous end-joining inhibitors.OncoTargets and therapy · 2018Article
- A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a 'self-cleaving' GFP-expression plasmid.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Psoriasiform skin disease in transgenic pigs with high-copy ectopic expression of human integrins α2 and β1.Disease models & mechanisms · 2017Article
- Generation of gene edited birds in one generation using sperm transfection assisted gene editing (STAGE).Transgenic research · 2017Article
- MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.Biotechnology and bioengineering · 2017Article
- Studying disorders of vertebrate iron and heme metabolism using zebrafish.Methods in cell biology · 2017Review
- CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development.Frontiers in neuroanatomy · 2017Article
- Intron-based genomic editing: a highly efficient method for generating knockin zebrafish.Oncotarget · 2015Review
- Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system.Cell research · 2015Article
- Efficient knockout of transplanted green fluorescent protein gene in medaka using TALENs.Marine biotechnology (New York, N.Y.) · 2014Article
- Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.Genome research · 2014Article
- Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair.Genome research · 2014Article
- Characterization of estrogen-responsive transgenic marine medaka Oryzias dancena germlines harboring red fluorescent protein gene under the control by endogenous choriogenin H promoter.Transgenic research · 2013Article
- PIG3 functions in DNA damage response through regulating DNA-PKcs homeostasis.International journal of biological sciences · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study focused on concatemer formation and integration pattern of transgenes in zebrafish embryos. A reporter plasmid based on enhanced green fluorescent protein (eGFP) driven by Cytomegalovirus (CMV) promoter, pCMV-pax6in-eGFP, was constructed to reflect transgene behavior in the host environment. After removal of the insertion fragment by double digestion with various combinations of restriction enzymes, linearized pCMV-pax6in-eGFP vectors were generated with different combinations of 5'-protruding, 3'-protruding, and blunt ends that were microinjected into zebrafish embryos. Repair of double-strand breaks (DSBs) was monitored by GFP expression following religation of the reporter gene. One-hundred-and-ninety-seven DNA fragments were amplified from GFP-positive embryos and sequenced to analyze the repair characteristics of different DSB end combinations. DSBs involving blunt and asymmetric protruding ends were repaired efficiently by direct ligation of blunt ends, ligation after blunting and fill-in, or removed by cutting. Repair of DSBs with symmetric 3'-3' protrusions was less efficient and utilized template-directed repair. The results suggest that non-homologous end joining (NHEJ) was the principal mechanism of exogenous gene concatemer formation and integration of transgenes into the genome of transgenic zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.