ArticlePloS one2010
The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Docking-Based Virtual Screening Identifies a Small-Molecule Inhibitor of the Translesion Synthesis REV7/REV3 Protein-Protein Interaction.ChemMedChem · 2026Article
- Probing hot spots of protein-protein interactions mediated by the safety-belt region of REV7.Structure (London, England : 1993) · 2024Article
- REV7: a small but mighty regulator of genome maintenance and cancer development.Frontiers in oncology · 2024Review
- REV7 in Cancer Biology and Management.Cancers · 2023Review
- Review
- REV7: Jack of many trades.Trends in cell biology · 2021Review
- Clathrin Light Chains: Not to Be Taken so Lightly.Frontiers in cell and developmental biology · 2021Review
- Genome-wide DNA copy number analysis and targeted transcriptional analysis of canine histiocytic malignancies identifies diagnostic signatures and highlights disruption of spindle assembly complex.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Article
- Dynamic feature of mitotic arrest deficient 2-like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment-maintaining phosphoprotein (CAMP).The Journal of biological chemistry · 2017Article
- Genome-wide association study revealed genomic regions related to white/red earlobe color trait in the Rhode Island Red chickens.BMC genetics · 2016Article
- The multifaceted roles of the HORMA domain in cellular signaling.The Journal of cell biology · 2015Review
- Rev7/Mad2B plays a critical role in the assembly of a functional mitotic spindle.Cell cycle (Georgetown, Tex.) · 2015Article
- MiCroKiTS 4.0: a database of midbody, centrosome, kinetochore, telomere and spindle.Nucleic acids research · 2015Article
- Molecular genetics and cellular features of TFE3 and TFEB fusion kidney cancers.Nature reviews. Urology · 2014Review
- Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.The Journal of cell biology · 2013Article
- A critical function of Mad2l2 in primordial germ cell development of mice.PLoS genetics · 2013Article
- Genomic imbalances in patients with a clinical presentation in the spectrum of Cornelia de Lange syndrome.BMC medical genetics · 2013Article
- Rev3, the catalytic subunit of Polζ, is required for maintaining fragile site stability in human cells.Nucleic acids research · 2013Article
- A novel binding protein of single-minded 2: the mitotic arrest-deficient protein MAD2B.Neurogenetics · 2012Article
- Novel functions of endocytic player clathrin in mitosis.Cell research · 2011Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough the mitotic arrest deficient protein MAD2B (MAD2L2) is thought to inhibit the anaphase promoting complex (APC) by binding to CDC20 and/or CDH1 (FZR1), its exact role in cell cycle control still remains to be established. METHODOLOGY/PRINCIPAL
findingsUsing a yeast two-hybrid interaction trap we identified the human clathrin light chain A (CLTA) as a novel MAD2B binding protein. A direct interaction was established in mammalian cells via GST pull-down and endogenous co-immunoprecipitation during the G2/M phase of the cell cycle. Through subsequent confocal laser scanning microscopy we found that MAD2B and CLTA co-localize at the mitotic spindle. Clathrin forms a trimeric structure, i.e., the clathrin triskelion, consisting of three heavy chains (CLTC), each with an associated light chain. This clathrin structure has previously been shown to be required for the function of the mitotic spindle through stabilization of kinetochore fibers. Upon siRNA-mediated MAD2B depletion, we found that CLTA was no longer concentrated at the mitotic spindle but, instead, diffusely distributed throughout the cell. In addition, we found a marked increase in the percentage of misaligned chromosomes. CONCLUSIONS/SIGNIFICANCE: Previously, we identified MAD2B as an interactor of the renal cell carcinoma (RCC)-associated protein PRCC. In addition, we found that fusion of PRCC with the transcription factor TFE3 in t(X;1)(p11;q21)-positive RCCs results in an impairment of this interaction and a concomitant failure to shuttle MAD2B to the nucleus. Our current data show that MAD2B interacts with CLTA during the G2/M phase of the cell cycle and that depletion of MAD2B leads to a marked increase in the percentage of misaligned chromosomes and a redistribution of CLTA during mitosis.
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